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juan_819鐵蟲(chóng) (初入文壇)
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[求助]
如何提取HMGR
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請(qǐng)問(wèn): 如何從植物中提取HMGR這個(gè)酶從而用以進(jìn)行酶活測(cè)定? 謝謝! |
專(zhuān)家顧問(wèn) (職業(yè)作家)
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專(zhuān)家經(jīng)驗(yàn): +426 |
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以前我是照這個(gè)方法做得 All enzymes assayed in this work were extracted from the hairy roots with an extraction buffer of 50 mM Tris–HCl, 10 mM β-mercaptoethanol, 1% (w/v) polyvinylpyrrolidone and pH7.5. The roots were ground in the extraction buffer (1 g FW/mL) for 5 min with a pestle and mortar on ice, followed by centrifugation at 14,000 rpm and 4°C for 30 min to obtain a solids-free extract. The protein content was determined by the Bradford method using bovine serum albumin as a standard. |

專(zhuān)家顧問(wèn) (職業(yè)作家)
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專(zhuān)家經(jīng)驗(yàn): +426 |

鐵蟲(chóng) (初入文壇)
鐵蟲(chóng) (初入文壇)
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