 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 1 | 1/1 | 返回列表 |
| 查看: 1802 | 回復: 0 | |||
Bessel木蟲 (正式寫手)
|
[交流]
Postdoctoral position in theoretical physics/biophysics (France)
|
|
Postdoctoral position in theoretical physics/biophysics Theory of the dynamics of DNA conformational changes. Comparison with Tethered Particle Motion experiments par Manoel Manghi - 15 octobre Contact : Manoel Manghi, manghi@irsamc.ups-tlse.fr Nicolas Destainville destain@irsamc.ups-tlse.fr Laboratoire de Physique Th??orique, Universit?? de Toulouse and CNRS, France, www.lpt.ups-tlse.fr The dynamics of DNA conformations induced by mechanical or biochemical constraints is a central issue for biologists and remains challenging for physicists. Indeed, on the biological point of view, it directly concerns the understanding of replication and transcription in vivo, which are out-of-equilibrium active processes. Tethered Particle Motion (TPM) experiments, which are good candidates for exploring the local DNA dynamics in presence of its partner proteins, are developed in L. Salom????s group of the IPBS in Toulouse [1]. Based on the tracking of a nano-particle attached to the free end of a DNA molecule immobilized by the other end on a surface, the TPM technique reveals the conformational dynamics of the DNA molecule [1, 2]. In particular, it has proved to provide a refined characterization of DNA-protein interactions. Recently, Salom????s group has succeeded in developing a single-DNA chip integrating the TPM technique that makes possible massive parallel measurements [3]. On the theoretical side, DNA dynamics is usually modeled using the classical Rouse dynamics (or Zimm one, if hydrodynamic interactions are taken into account) [4], where DNA is seen as a simple polymer without internal structure. Although this approach is valid on length scales larger than 100 base-pairs, it fails at the smaller scale of interest, where taking the double helical structure into account becomes essential. Moreover, the dynamics becomes much more complex as soon as counterions, hydrodynamics interactions, external constraints (external or induced by partner proteins) are considered. The theoretical work, to which the candidate will contribute, consists in elucidating a number of issues concerning the intra-chain DNA dynamics which might be observable in TPM experiments. The candidate will focus on the influence of local elastic properties (which varies with the local base-pairing state [5]) on the looping dynamics. For instance, it has recently been proposed [6] that the extremely slow closure of DNA denaturation bubbles (local DNA segments where base-pairs are broken), observed in experiments, is essentially due to a metastable barrier associated to the bending energy stored in the bubble (which is much more flexible than the double-helix segments). This metastable state is relaxed once the double-helix strands have diffused towards an aligned state. Such activation barriers might also play a central role in protein-DNA complexation, which are kinetically controlled. Types of questions that await answers are : How does the DNA local elasticity modify reaction rates ? How does the geometric constraint induced by proteins on DNA in synaptic complexes contribute to reactions rates and equilibrium constants ? Is the formation of local bubbles in constrained DNA a limiting step ? The experimental studies by TPM on the Xer-dif synapse (collaboration with biologists F. Cornet and P. Rousseau [2]) and the DNA looping by Lac repressor might be a challenging context for such a theoretical study. The theoretical methods used in this study will be both standard analytical tools of non-equilibrium statistical physics (Langevin equation, mean first passage time, hydrodynamic interactions) and polymer physics (Worm-Like Chain model, transfer matrices), and numerical simulations (Kinetic Monte-Carlo, Brownian dynamics) [2, 7]. The candidate will join the Statistical Physics group of the Theoretical Physics Laboratory and participate to the ??TPM-on-a-chip?? ANR project in which is granted this two-years postdoctoral position starting on 1st semester of 2013. An effort will be made on the comparison with experiments done by the biophysicists involved in this pro ject (Salom????s group at IPBS and R. Phillips?? group of California Institute of Technology). The salary will be of about 2040? per month (2650$) (before income taxes, after social taxes) and the candidate will benefit from the French health insurance (S??curit?? Sociale) and employment benefits. Applicants should send their application letter along with their Curriculum Vitæ and publication list before March 1st, 2013. Recommendation letters are welcome. References : [1] N. Pouget, C. Turlan, N. Destainville, L. Salom??, M. Chandler. IS911 transpososome assembly as analysed by tethered particle motion, Nucl. Acids Res. 34 4313 (2006). [2] M. Manghi, C. Tardin, P. Rousseau, J. Baglio, L. Salom??, N. Destainville, Probing DNA conformational changes with high temporal resolution by tethered particle motion, Phys. Biol. 7 046003 (2010). [3] T. Plenat, C. Tardin, P. Rousseau, L. Salom??, High-throughput single-molecule analysis of DNA-protein interactions by tethered particle motion, Nucl. Acids Res. 40 e89 (2012). [4] M. Doi, S.F. Edwards, The theory of polymer dynamics, Clarendon Press, 1986. [5] J. Palmeri, M. Manghi, N. Destainville, Thermal denaturation of fluctuating DNA driven by bending entropy, Phys. Rev. Lett. 99 088103 (2007). [6] A.K. Dasanna, N. Destainville, J. Palmeri, M. Manghi, Strand diffusion-limited closure of denaturation bubbles in DNA, EPL 98 38002 (2012). [7] M. Manghi, X. Schlagberger, Y.-W. Kim, R.R. Netz, Hydrodynamics effects in driven soft matter, Soft Matter 2 653 (2006). |
回復此樓» 猜你喜歡
310求調(diào)劑
已經(jīng)有6人回復
-
面上5B能上會嗎？
已經(jīng)有5人回復
-
279求調(diào)劑
已經(jīng)有3人回復
-
材料與化工272求調(diào)劑
已經(jīng)有17人回復
-
材料工程專碩求調(diào)劑
已經(jīng)有3人回復
-
337求調(diào)劑
已經(jīng)有5人回復
-
材料與化工328分調(diào)劑
已經(jīng)有9人回復
-
318一志愿吉林大學生物與醫(yī)藥 求調(diào)劑
已經(jīng)有5人回復
-
一志愿南開大學0710生物學359求調(diào)劑
已經(jīng)有4人回復
-
求調(diào)劑
已經(jīng)有6人回復
找到一些相關(guān)的精華帖子，希望有用哦~
Postdoctoral position the Goethe-University of Frankfurt
已經(jīng)有3人回復
- postdoctoral position for Lithium battery in Canada
已經(jīng)有4人回復
- Postdoctoral Position in Canada
已經(jīng)有8人回復
- 個人上周招聘資訊勁爆分享（MAY 23-29）
已經(jīng)有3人回復
- 【原創(chuàng)】Postdoctoral Position at the University of Virginia
已經(jīng)有5人回復
- 【原創(chuàng)】行政人員說在等 postdoctoral position to be approved，什么意思
已經(jīng)有8人回復
- 【轉(zhuǎn)載】Postdoctoral position in University of Queensland
已經(jīng)有15人回復
- 【原創(chuàng)】postdoctoral position in University of Minnesota
已經(jīng)有3人回復
- Postdoctoral Position at Nanyang Technological University
已經(jīng)有3人回復
- 【轉(zhuǎn)載】博后招聘--Postdoctoral Position at UC Davis
已經(jīng)有7人回復
- Postdoctoral Position
已經(jīng)有8人回復
點擊這里搜索更多相關(guān)資源科研從小木蟲開始，人人為我，我為人人
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 Bessel 的主題更新
| 1 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 材料工程專碩求調(diào)劑 +3 | hyl3153942 2026-03-29 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 一志愿河北工業(yè)大學0817化工278分求調(diào)劑 +10 | jhybd 2026-03-23 | 15/750 |
|
|
|
[考研] 一志愿北京工業(yè)大學，324分求調(diào)劑 +6 | 零八# 2026-03-28 | 6/300 |
|
|
|
[考研] 求調(diào)劑 +4 | QiMing7 2026-03-25 | 5/250 |
|
|
|
[考研] 一志愿211 初試270分 求調(diào)劑 +8 | 谷雨上岸 2026-03-23 | 9/450 |
|
|
|
[考研] 346求調(diào)劑 一志愿070303有機化學 +3 | 蘿卜燉青菜 2026-03-28 | 3/150 |
|
|
|
[考研] 0703一志愿9，初試成績：338，四六級已過，有科研經(jīng)歷，求調(diào)劑！ +4 | Zuhui0306 2026-03-25 | 4/200 |
|
|
|
[考研] 311求調(diào)劑 +9 | lin0039 2026-03-26 | 9/450 |
|
|
|
[有機交流]
高溫高壓反應(yīng)求助
 10+4
|
chibby 2026-03-25 | 4/200 |
|
|
|
[考研] 266求調(diào)劑 +11 | 陽陽哇塞 2026-03-27 | 12/600 |
|
|
|
[考研] 308求調(diào)劑 +7 | 墨墨漠 2026-03-25 | 7/350 |
|
|
|
[考研] 求調(diào)劑 +3 | 劉柯@ 2026-03-24 | 4/200 |
|
|
|
[考研] 315調(diào)劑 +4 | 0860求調(diào)劑 2026-03-26 | 5/250 |
|
|
|
[考研] 359求調(diào)劑 +4 | 王了個楠 2026-03-25 | 4/200 |
|
|
|
[考研] 調(diào)劑 +4 | 柚柚yoyo 2026-03-26 | 4/200 |
|
|
|
[考研] 中國科學院深圳先進技術(shù)研究院-光纖傳感課題組招生-中國科學院大學、深圳理工大學聯(lián)培 +5 | YangTyu1 2026-03-26 | 5/250 |
|
|
|
[考研] 282求調(diào)劑 +3 | wcq131415 2026-03-24 | 3/150 |
|
|
|
[考研] 086003食品工程求調(diào)劑 +6 | 淼淼111 2026-03-24 | 6/300 |
|
|
|
[考研] 318求調(diào)劑 +3 | plum李子 2026-03-23 | 3/150 |
|
|
|
[考研]
 材料考研調(diào)劑生
+3
|
黃粱一夢千年 2026-03-24 | 3/150 |
|