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123456qi木蟲 (職業(yè)作家)
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[求助]
請前輩幫看看這是什么意思!急!急!急。ń饚挪欢啵堈徑猓
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本人欲做單晶培養(yǎng),以前從未接觸過這方面的內(nèi)容,在文獻(xiàn)上看到相關(guān)培養(yǎng)方法,不知道是什么意思,請幫助解釋一下! METHOD: vapor diffusion, sitting drop DETAILS: 12% PEG4000, 15% isoproponal, 25mM MOPS, PH6.5, 150 mM NaCl, 2mM DTT, vapor diffusion, sitting drop. 相關(guān)試劑都是什么啊,百分比濃度及摩爾濃度是怎么回事(溶劑是什么啊?) ,可否告知具體怎么操作啊?萬分感謝! |
木蟲 (正式寫手)

木蟲 (職業(yè)作家)
木蟲 (職業(yè)作家)
鐵桿木蟲 (著名寫手)
木蟲 (職業(yè)作家)
木蟲 (職業(yè)作家)
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Compounds were dissolved in Me2SO to a final concentration of 20 mM and then mixed with c-Met protein to a finalMe2SOconcentration of 4% before crystallization. Crystals of compounds were grown from 12% polyethylene glycol 6000, 1.0 M LiCl2, and 0.1 M sodium citrate, pH 5.0. Large crystals suitable for data collection were obtained by microseeding. 這段是什么意思啊,請前輩指教!小弟不甚感激! |
木蟲 (職業(yè)作家)

鐵桿木蟲 (著名寫手)
木蟲 (職業(yè)作家)
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