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smallsv金蟲 (正式寫手)
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[求助]
求助一段翻譯,謝謝
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An oligonucleotide linker, to fuse the truncated TNFR with the human IgG1 Fc fragment. This linker was created by PCR (polymerase chain reaction) amplification using two primers, one having the sequence CCCCAGCTGAAGGGAGCACTGGCG ACGAGCCCAAATCTTGTGACAAAACTC (nucleotides 833-883 of SEQ ID NO:3) which encodes the 3' end of the truncated TNF receptor and the 5' end of human IgG1, and the other having the sequence CGGTACGTGCTGTTGTTACTGC (SEQ ID NO:5), an antisense sequence encoding nucleotides 257-237 of human IgG1. The template for this reaction was pIXY498. The reaction product was digested with Pvu2 and Sty1, and a 115 bp fragment was isolated. |

金蟲 (小有名氣)
| 一個(gè)寡核苷酸連接器與去頂端的和人體免疫球蛋白G1核仁纖維中心碎片的腫瘤壞死因子受體融合。連接器由兩個(gè)引子的PRC(聚合酶鏈反應(yīng))放大產(chǎn)生。兩個(gè)引子一個(gè)序列是CCCCAGCTGAAGGGAGCACTGGCG ACGAGCCCAAATCTTGTGACAAAACTC (序列號ID NO:3,883-883核苷酸),它的3‘端為縮短了的腫瘤壞死因子受體,5’端為人體免疫球蛋白G1,另一個(gè)引子序列是CGGTACGTGCTGTTGTTACTGC (SEQ ID NO:5),是人體免疫球蛋白G1的反序列編碼核苷酸257-237。反應(yīng)模板是pIXY498。反應(yīng)產(chǎn)物摘要為Pvu2 和 Sty1,一個(gè)115bp碎片獨(dú)立開來。 |


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