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[求助]
求一段英譯中，萬分感謝
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In the unfolding process, it was observed that ovalbumin and αlactalbumin followed a three state transition pathway involving an intermediate state having high surface hydrophobicity. The intermediate state has also been characterized by circular dichroism spectroscopy, and it was found that the intermediate retained almost the same secondary structure as the native proteins, and therefore it can be referred to as molten globule state. The refolding process was monitored using fluorescence and circular dichroism spectroscopy, and it was observed that the refolding of αlactalbumin was reversible and proceeded through the accumulation of similar type of ntermediates as observed during its unfolding pathway. However, on refolding from the guanidine hydrochloridedenatured state, ovalbumin reached a different folded state. |
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xiangyi007
金蟲 (小有名氣)
- 翻譯EPI: 12
- 應助: 0 (幼兒園)
- 金幣: 900
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- 帖子: 151
- 在線: 45.8小時
- 蟲號: 1829097
- 注冊: 2012-05-22
- 性別: GG
- 專業(yè): 結(jié)構(gòu)化學
【答案】應助回帖
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愛與雨下: 金幣+1 2012-12-21 18:36:03
qwky2010: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 08:46:31
愛與雨下: 金幣+1 2012-12-21 18:36:03
qwky2010: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 08:46:31
| 在展開過程中觀察到，卵清蛋白和乳清蛋白遵循三個狀態(tài)轉(zhuǎn)變路線包括一個具有強表面疏水性的中間態(tài)。中間態(tài)用圓二色光譜表征，還發(fā)現(xiàn)中間態(tài)保留了大多數(shù)與天然蛋白質(zhì)一樣的次級結(jié)構(gòu)，因此可以歸為熔球態(tài)。重折疊過程在熒光和圓二色光譜下觀察，也觀察到重乳清蛋白折疊過程是可逆的，是在其展開過程中觀察到的簡單類型中間態(tài)的積累。但是，從鹽酸胍態(tài)的重折疊，卵清蛋白形成一種新的折疊態(tài)。 |
2樓2012-12-21 17:28:49
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