| 2 | 1/1 | 返回列表 |
| 查看: 925 | 回復(fù): 1 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ,點(diǎn)擊這里進(jìn)行查看 | |||
yind1014鐵蟲(chóng) (初入文壇)
|
[求助]
關(guān)于HPLC分析時(shí)色譜條件的選擇的一段話 求翻譯
|
||
|
Analytical separation was carried out on a Jasco PV- 1580 HPLC unit using a Supelco 516 C18 column (15 cm × 4.6 mm, 5μm) at room temperature. The detector used was a Jasco UV-1570 UV–vis spectrophotometer set at 254 nm and the volume of sample injected was 20 L. The aqueous phase was prepared by weighing 3.8 g ammonium acetate and dissolving in 1 L water and its pH adjusted to 4.4 using acetic acid. The mobile phase used was methanol–acetate buffer (pH 4.4) (35:65, v/v) for 9 min, after which it was changed to methanol–acetate buffer (pH 4.4) (50:50, v/v). |
版主 (文壇精英)
米米
| 分析分離在Jasco PV-1580 液相色譜里進(jìn)行,室溫,Supelco 516 C18 柱子。 探測(cè)器是Jasco UV-1570紫外-可見(jiàn)光光譜儀,用254nm,注入樣品是20升。水相制備由3.8克醋酸銨溶于一升水,用醋酸調(diào)節(jié)PH至4.4. 流動(dòng)相用甲醇-醋酸緩沖液 (pH 4.4) (35:65體積比),之后變?yōu)榧状?醋酸緩沖液(pH 4.4) (50:50體積比). |
| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿華東師范大學(xué)有機(jī)化學(xué)專業(yè),初試351分,復(fù)試被刷求調(diào)劑! +3 | 真名有冰 2026-03-29 | 4/200 |
|
|---|---|---|---|---|
|
[考研] 085600 材料與化工 329分求調(diào)劑 +11 | Mr. Z 2026-03-25 | 11/550 |
|
|
[考研] 0703化學(xué)調(diào)劑,求導(dǎo)師收 +9 | 天天好運(yùn)來(lái)上岸?/a> 2026-03-24 | 10/500 |
|
|
[考研] 學(xué)碩274求調(diào)劑 +9 | Li李魚(yú) 2026-03-26 | 9/450 |
|
|
[考研] 322求調(diào)劑 +7 | 宋明欣 2026-03-27 | 7/350 |
|
|
[考研] 22408 359分調(diào)劑 +4 | Qshers 2026-03-27 | 5/250 |
|
|
[考研] 求調(diào)劑 +7 | 爭(zhēng)取九點(diǎn)睡 2026-03-28 | 8/400 |
|
|
[考研] 085600 286分 材料求調(diào)劑 +7 | 麻辣魷魚(yú) 2026-03-27 | 8/400 |
|
|
[考研] 材料292調(diào)劑 +12 | 橘頌思美人 2026-03-23 | 12/600 |
|
|
[考研] 085600材料與化工調(diào)劑 +10 | A-哆啦Z夢(mèng) 2026-03-23 | 16/800 |
|
|
[考研] 333求調(diào)劑 +3 | question挽風(fēng) 2026-03-23 | 3/150 |
|
|
[考研] 315調(diào)劑 +4 | 0860求調(diào)劑 2026-03-26 | 5/250 |
|
|
[考研]
|
鐘llll 2026-03-26 | 4/200 |
|
|
[考研] 333求調(diào)劑 +6 | wfh030413@ 2026-03-23 | 6/300 |
|
|
[考研] 081200-11408-276學(xué)碩求調(diào)劑 +3 | 崔wj 2026-03-26 | 3/150 |
|
|
[考研] 材料考研求調(diào)劑 +3 | Dendel 2026-03-23 | 6/300 |
|
|
[考研] 機(jī)械學(xué)碩總分317求調(diào)劑。。! +4 | Acaciad 2026-03-25 | 4/200 |
|
|
[考研] 290分調(diào)劑求助 +3 | 吉祥止止陳 2026-03-25 | 3/150 |
|
|
[考研] 生物學(xué)學(xué)碩求調(diào)劑 +7 | 小羊睡著了? 2026-03-23 | 10/500 |
|
|
[考研] 335求調(diào)劑 +4 | yuyu宇 2026-03-23 | 5/250 |
|