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your2007至尊木蟲 (著名寫手)
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[求助]
求一段英譯中，萬分感謝
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| Using Surface Plasmon Resonance (SPR) it has been shown that the fine structure of the anionic polysaccharide pectin strongly influences its interfacial interaction with a j-casein layer coated onto a gold surface (via a dextran linker) in the pH range 3.5–6.8, with the highest SPR signal being observed for pectin with the lowest charge density tested (a degree of methylesterification (DM) around 90%).Furthermore, the Brownian motions of j-casein coated polystyrene beads (used to provide calciumfree ‘model casein micelles’) were studied in pectin solutions using Diffusing Wave Spectroscopy (DWS) and microscopy, and were compared with measurements made on naked beads. At every pH value studied (with the exception of 3.5), bridging of the protein-covered probe particles was observed for pectins of both DM 28 and DM 78. However, no aggregated complexes were found in these model casein micelle systems when pectin of an unusually high DM was used (90%). It was hypothesised that having a limited number of binding regions of spatially limited extent maximises the number of chains binding to the protein layer (as found with the SPR measurement), encourages the formation of loops and trains, and additionally limits the potential for destabilisation via bridging. |
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【答案】應(yīng)助回帖
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your2007: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 08:48:08
your2007: 金幣+30, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 08:48:08
| 使用表面等離子體共振（SPR），已經(jīng)指出在pH范圍3.5-6.8，陰離子多糖果膠的精細(xì)結(jié)構(gòu)強(qiáng)烈影響其與覆蓋到金表面上的j-酪蛋白層之間的界面相互作用（通過葡聚糖連接器），觀察到果膠最高的SPR信號(hào)，檢測(cè)到最低的電荷密度（甲酯化程度（DM）在90％左右）。此外，在果膠溶液中，對(duì)被聚苯乙烯珠覆蓋的j-酪蛋白的布朗運(yùn)動(dòng)（用來提供無鈣模型酪蛋白膠束）進(jìn)行了研究，采用擴(kuò)散波光譜（DWS）和顯微鏡，并與裸珠的測(cè)量結(jié)果進(jìn)行了對(duì)比。在每個(gè)pH值（除去3.5），研究果膠DM28和DM78，觀察到蛋白質(zhì)包覆的探針顆粒的橋連。然而，在這些模型酪蛋白體系，當(dāng)使用一個(gè)不尋常的高DM的果膠時(shí)（90％），沒有發(fā)現(xiàn)凝集的配合物。有人假設(shè)，空間有限的程度上具有有限數(shù)量的橋連區(qū)域可以使橋連蛋白質(zhì)層的鏈達(dá)到最大化（SPR測(cè)量發(fā)現(xiàn)），鼓勵(lì)循環(huán)和行列的形成，并且還限制了由于橋連不穩(wěn)定的可能性。 |
2樓2012-12-23 22:04:02
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