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your2007至尊木蟲 (著名寫手)
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求一段英譯中，萬(wàn)分感謝
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The experimental results demonstrate that two structurally distinct species exist in the conformational transition of apo-BLA induced by low pH. One species populates at pH 3.0 characterized as a monomeric molten globule state and the other accumulates at pH 4.0–4.5 which is a partially folded dimer. Oleic acid promotes the formation of the dimeric intermediate at pH 4.0 and 7.0, but increases the content of molten globule state remarkably at pH 3.0 compared with that in the absence of oleic acid, indicating that oleic acid at pH 3.0 plays a different role from those at pH 4.0 and 7.0. Our data provide insight into the mechanism of pH-dependent and oleic acid-dependent structural changes and oligomerization of α-lactalbumin, and will be helpful to the understanding of the apoptosis-inducing function of multimeric α-lactalbumin in which oleic acid is a necessary cofactor. |
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【答案】應(yīng)助回帖
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your2007: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 11:41:36
your2007: 金幣+20, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2012-12-24 11:41:36
| 實(shí)驗(yàn)結(jié)果表明在低PH誘導(dǎo)的脂蛋白酶構(gòu)象遷移中存在兩個(gè)結(jié)構(gòu)上明顯不同的種類。一種在PH為3.0時(shí)以單體熔球狀態(tài)出現(xiàn)，而另一種則在PH4.0到4.5之間以部分折疊二聚物存在。十八烯酸在PH4.0到7.0時(shí)促進(jìn)二聚中間體的形成，但是在PH為3.0時(shí)，與沒有十八烯酸相比，應(yīng)用十八烯酸能明顯增加熔球狀態(tài)的容量。這表明十八烯酸在PH3.0與PH4.0到7.0之間所起的作用不同。數(shù)據(jù)表明深刻理解PH依賴與十八烯酸依賴的結(jié)構(gòu)變化和α-乳清蛋白的低聚反應(yīng)等的機(jī)理，對(duì)于理解十八烯酸做輔助因子的多聚α-乳清蛋白的細(xì)胞誘導(dǎo)凋亡功能是很有幫助的。 |
2樓2012-12-24 11:15:30
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