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nalanyuhang新蟲 (初入文壇)
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[求助]
水氯仿甲醇提取組織代謝物 已有1人參與
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| 實驗中要用CE/MS測腫瘤組織中代謝物,代謝物提取過程如下:組織加20ul/mg甲醇勻漿后,加水,氯仿(體積比5:2:5),離心取上清。。。。!,F(xiàn)在想知道的是加水、甲醇、氯仿的目的各是什么?離心后上清的水相中有甲醇嗎,還是甲醇在氯仿相中?文獻中只要水溶液是因為小代謝物大多水溶嗎,這樣只要水相結(jié)果會精確嗎? 在線等大家一起交流指導,感激不盡!! |

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Samples were vortexed for 1 min, left for 10 min on ice for partition and centrifuged (2,0009g, 10 min, 4 C) to obtain a clear biphasic mixture, upper aqueous/polar phase and lower organic/apolar phase. The upper polar phase was collected and the lower organic layer was carefully recovered using a positive-displacement Microman pipette (Gilson, Middleton, WI, USA), without disturbing the layer between the two phases containing protein and small tissue debris. 組織和蛋白沉淀在中間層 |

新蟲 (初入文壇)
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