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xxjjtt新蟲 (初入文壇)
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[求助]
Origami™ B(DE3) 菌株 是否有l(wèi)acZ活性？
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如題； 我買的Origami™ B(DE3) Competent Cells，介紹里面說是一株BL21的LacZY基因缺失菌株。小弟想知道，該菌株的lacZ活性還存在不？ 附Origami™ B(DE3) Competent Cells的介紹和基因型： Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F S (r m ) -ompT hsdB B-B-galdcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (Kan , Tet ) |
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xxjjtt
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- 蟲號: 1505147
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- 專業(yè): 微生物資源與分類學(xué)
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沒有人回啊， 今天我收到了millipore客服的回信，內(nèi)容如下： The Origami™ B(DE3) strain with cat #70837 is a derivative of BL21. It carries the lacY1 mutation that although it hasn't been quite well characterized, we believe it has a deletion mutation of both lacY and lacZ. 應(yīng)該是一株lacZ缺失菌株。希望能幫到有一樣疑問的同學(xué) |
2樓2013-01-17 16:21:20
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