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[求助]
Tryton是什么東西?
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| 看一篇文獻寫PCR體系中寫到: 2.5 mM MgCl 2 , 0.1% Tryton (Promega, Madison, Wis.),其中的Tryton是什么意思? |
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查到你的那篇文獻了 說實話,我感覺是他寫錯了,當然我以前也沒做個這個pcr 個人認為應該是triton,具體的理由就是,我剛去翻看了另外一篇文獻,里邊有這么一段話 “Several approaches have been proposed to overcome this problem including additives usage in different PCR procedures (2, 6). Different additives were utilized to amplify GC-rich regions including betaine (N,N,N- trimethylglycin), dimethyl sulfoxide (DMSO), formamide, glycerin, glycerol, dithiothreitol (DTT), Bovine Serum Albumin (BSA), non-ionic detergents such as Triton X-100, nucleotide analogs 7-deaza dGTP, ethylene glycol and 1, 2-propanediol, and reducing agents such as β-mercapto-ethanol (1–9). These additives not only prevent secondary structure formation of both templates and primers, but also increase the annealing chance of primers to plate strands at temperatures lower than the melting temperature of primers (1, 3, 5, 7, 9). ” 文章地址 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558226/ |
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