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黃赤金蟲 (正式寫手)
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Cell viability was determined by the MTT assay. Briefly, cells were plated in 96-well flat-bottomed plates at 5 × 10 3 cells per well and allowed to grow overnight prior to exposure to MCNs, DOX, or DOX@MCNs with different concentrations, followed by irradiation with red light. After 24 or 48 h of further incubation, the MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide) was added for 4 h at 37 ° C to allow conversion of MTT to a purple formazan product by active mitochondria. Then the formazan product was dissolv ed in DMSO and quantifi ed by absorption spectrophotometry at 490 nm on an enzyme-labeled instrument (SUNOSTIK SPR-960). |
榮譽版主 (職業(yè)作家)
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利用MTT 法測定細胞活性 首先,細胞被接種到96孔平底板,細胞濃度調(diào)整到5 ×103個/孔,培養(yǎng)過夜,然后添加不同濃度的MCNs或DOX, 或者同時添加DOX和MCNs ,最后用紅光照射。在培養(yǎng)箱中培養(yǎng)24h或者48h后,添加MTT至96孔板,每孔10-20ul,然后放置于37℃二氧化碳培養(yǎng)箱中4h,讓MTT在活細胞中的活性線粒體作用下充分形成藍紫色的甲瓚結(jié)晶。最后,每孔添加150ulDMSO,將藍紫色結(jié)晶溶解,利用吸光光度法,用酶標儀在490nm波長下測定其OD值(吸光度值)。 正好我最近在做MTT法,英文里面有些沒交代清楚,我已經(jīng)在翻譯里給加上了。希望能幫到你! ![]() |

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