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嗰嘰嗰嘰金蟲 (小有名氣)
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[求助]
摘要翻譯,謝謝
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A novel lumbricin-like antimicrobial peptide named lumbricin-PG was isolated from skin secretions of the earthworm, Pheretima guillelmi (Michaelsen), using a procedure of one step Sephadex G-50 gel filtration and one step C8reversephase high-performance liquid chromatography (RP-HPLC). Its aminoacid sequence was determined as FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEM-PAEGGHIEP by Edman degradation combined with cDNA cloning and mass spectrometry analysis. The cDNA encoding lumbricin-PG was cloned by cDNA library screening. The predicted protein from the cDNA sequence was composed of 73 amino acid residues including a mature lumbricin-PG and predicted signal peptide. It showed similarity with lumbricin antimicrobial peptide from the earthworm, Lumbricus rubellus by BLAST search. Purified lumbricin-PG exerted potential antimicrobial activities against bacteria and fungi; it showed weak hemolysis activity against human and rabbit red cells. |

禁蟲 (知名作家)
| 一種被稱作蚯蚓素-PG的新蚯蚓狀抗菌肽從一種稱作廉環(huán)毛蚓的蚯蚓表皮分泌物中分離出來,分離過程是通過一步交聯(lián)葡聚糖G-50溶膠過濾以及一步C8逆相高效液相層析法(簡稱:RP-HPLC)實(shí)現(xiàn)的。它的氨基酸序列被Adman通過結(jié)合cDNA克。╟DNA編碼通過cDNA庫篩選的方法進(jìn)行)和質(zhì)譜分析確定為FSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNN EGSPIFEM-PAEGGHIEP,。目前認(rèn)為cDNA蛋白質(zhì)序列由73個(gè)氨基酸殘基組成,包括一種自然的蚯蚓素-PG以及信號肽。結(jié)果顯示這種信號肽與BLAST研究組發(fā)現(xiàn)的粉正蚓體內(nèi)的抗菌肽相似。提純后的蚯蚓素-PG具有潛在的抗活性(包括真菌和細(xì)菌);它對人體以及兔子紅細(xì)胞具有弱的溶血活性。 |
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