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冰楓de夢(mèng)

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[求助] 英文摘要修改，中英文摘要都有了請(qǐng)大俠幫忙修下

探究Rho激酶抑制劑Y-27632對(duì)間充質(zhì)干細(xì)胞（mesenchymal stem cells,MSCs）C3H10T1/2增殖和成脂分化的影響. 培養(yǎng)C3H10T1/2細(xì)胞系，分為對(duì)照組、成脂誘導(dǎo)組和成脂誘導(dǎo)處理組（Y-27632+成脂誘導(dǎo)），MTT檢測(cè)細(xì)胞增殖情況，油紅O染色異丙醇萃取法檢測(cè)細(xì)胞成脂分化情況，半定量RT-PCR檢測(cè)過氧化物酶體增殖物激活受體γ(peroxisome proliferator activiated receptor γ, PPARγ)和CCAAT增強(qiáng)子結(jié)合蛋白α(CCAAT enhancer binding protein α, C/EBPα)基因表達(dá). 結(jié)果表明，Y-27632能夠顯著抑制C3H10T1/2細(xì)胞的增殖，并呈一定的濃度依賴性；高濃度Y-27632對(duì)C3H10T1/2細(xì)胞成脂分化具有顯著抑制作用（P<0.05）；半定量RT-PCR結(jié)果顯示，成脂誘導(dǎo)處理組PPARγ和C/EBPα表達(dá)量在第3 d、5 d、7 d顯著低于成脂誘導(dǎo)組（P<0.05）. 綜上所述，Y-27632能夠抑制C3H10T1/2細(xì)胞增殖與成脂分化.

To investigate the effect of Rho kinases inhibitor Y-27632 on proliferation and adipogenic differentiation of C3H10T1/2 mesenchymal stem cell. C3H10T1/2 cell were cultured in DMEM medium with 10% fetal bovine serum at 37℃ in 5% CO2 incubator. The experimental divided into control group, adipogenic group, adipogenic with Y-27632 group. The effects of Y-27632 on proliferation were detected by MTT. The adipogenic differentiation was characterized by oil red O staining. The differentiation of C3H10T1/2 cell to adipocyte was verified by expression of genes PPARγ and C/EBPα through RT-PCR. The results showed that Y-27632 obviously inhibited the proliferation of C3H10T1/2 cell in a dose-dependengt manner(P<0.05). High concentrations of Y-27632 significantly inhibited adipogenic differentiation of C3H10T1/2 cell (P<0.05). RT-PCR showed that the expression of PPARγ and C/EBPα in the 3rd, 5th day and 7th day were significantly inhibited by Y-27632 compared to the control (P<0.05). In summary, Y-27632 could inhibit the proliferation and adipogenic differentiation of C3H10T1/2 cell.

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愛拼才會(huì)贏

1樓 2013-04-27 15:10:37

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

chenjung0825

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【答案】應(yīng)助回帖

★ ★ ★
冰楓de夢(mèng)(phu_grassman代發(fā)): 金幣+3, 翻譯EPI+1, Thank you for yoru suggestion 2013-06-29 12:24:10

這個(gè)摘要需要改一改，給的細(xì)節(jié)太多了，反而目的和意義沒凸顯出來。另外，你的英文摘要第一句不是一個(gè)完整的句子。

贊一下

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2樓2013-04-27 16:18:19