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Spore suspension of plant pathogens were obtained from their respective 7-day-old cultures and mixed with sterile distilled water to obtain the homogenous spore suspension of 2 ×10^6 spore/ml. 500 l of spore suspension of each fungi was added to Petri plates that contained different concentrations of extracts (0, 500, 1000 and 1500 ppm) and spread with sterile lob. After 20 h, spore germination was counted around a limited area (2 cm diameter) under the microscope |
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