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aft.1988金蟲 (初入文壇)
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[求助]
如何判斷動態(tài)猝滅還是靜態(tài)猝滅
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| 做藥物與HSA的熒光實(shí)驗(yàn),Ksv隨著溫度的升高增大,但是Kq卻達(dá)到10的12次方遠(yuǎn)大于10的10次冪,怎么判斷究竟是動態(tài)猝滅還是靜態(tài)猝滅?有相應(yīng)的參考文獻(xiàn)嗎?紫外能夠輔助判斷靜態(tài)猝滅還是動態(tài)猝滅嗎?求助各位高手! |
專業(yè) |
金蟲 (初入文壇)
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剛看到一篇類似的文獻(xiàn),人家是這樣解釋的:囧~~ The plots show a good linear relationship within the investigated concentrations. The results showed that Kq were much greater than the limiting diffusion rate constant of the biomolecule (2.0 × 10-10L mol−1S−1), which indicated that the probable quenching mechanism of BSA–IMTZ interactions was initiated by complex formation rather than by dynamic collision. While the results also showed that the values of Stern–Volmer quenching constants KSV and Kq increased with increasing tem-perature, it indicated that the probable quenching mechanism of BSA–IMTZ interactions was initiated by dynamic collision [19,20] .In other words, the fluorescence quenching of BSA results from complex formation, while that dynamic collision could be not neg-ligible. |
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