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金獅水友至尊木蟲(chóng) (著名寫(xiě)手)
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[求助]
英譯漢---生物傳感---求翻譯--有效期3天---4---
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To minimize the effect of RNase on the stability of miRNAs, all glassware and centrifuge tubes were autoclaved, all solutions were treated with diethylpyrocarbon- ate, and all surfaces were decontaminated with RNaseZap (Ambion, TX). RNase-free ultrapure water was used throughout. Reference values of miRNA expression levels in the samples were obtained by using a commercial miRNA qRT- PCR kit from Agilent Technologies (Santa Clara, U.S.). |

金蟲(chóng) (小有名氣)
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為了減小RNA酶對(duì)于miRNA穩(wěn)定性的效果,所有的玻璃器皿和離心管都要高溫滅菌,所有的溶液都要用焦碳酸二乙酯 處理,所有的表面要用RNA酶的酵母聚糖激活血漿(Ambion, TX)凈化處理。全程使用無(wú)RNA酶的超純化水。樣品中miRNA表達(dá)水平的參考數(shù)值是由安捷倫科技公司(圣克拉拉,美國(guó))生產(chǎn)的一種商業(yè)的miRNA實(shí)時(shí)定量聚合酶鏈反應(yīng)設(shè)備所獲得。 |
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