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1049131934銅蟲 (小有名氣)
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[交流]
DNA聚合酶 已有1人參與
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| 有沒有大師指導(dǎo)DNA聚合酶Ⅲ的詳細(xì)的組裝過程。就是有那些個因子或者蛋白組裝成全酶的過程 |
金蟲 (正式寫手)
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E.coli. 1.DnaA先綁到叫dnaA boxes的起始位點上,然后與RNA聚合酶和HU蛋白相互作用。前者是用來合成起始DNA復(fù)制的引物,后者是為了讓dnaA box左端的的部分雙鏈融解為單鏈。 2.然后DnaB蛋白綁到開放的復(fù)合物上(融解為單鏈的區(qū)域)上,給引物綁到DNA模板鏈提共了方便。DnaB也有解旋酶活性,可以使雙鏈變?yōu)閱捂。形成的單鏈為了避免又成為雙鏈,往往會綁上單鏈結(jié)合蛋白(SSB). 3.DNA 核心酶通過由兩個beta亞基組成的滑動環(huán)結(jié)合到DNA模板鏈上進行復(fù)制。真核生物的滑動環(huán)由三個beta亞基組成。 |

金蟲 (正式寫手)
| The pol III holoenzyme is double-headed, with two core polymerases attached through two τ-subunits to a g complex. One core is responsible for (presumably) continuous synthesis of the leading strand, the other performs discontinuous synthesis of the lagging strand. The g complex serves as a clamp loader to load the b clamp onto a primed DNA template. Once loaded, the b clamp loses affi nity for the g complex and associates with the core polymerase to help with processive synthesis of an Okazaki fragment. Once the fragment is completed, the b clamp loses affi nity for the core polymerase and associates with the g complex, which acts as a clamp unloader, removing the clamp from the DNA. Then it can recycle to the next primer and repeat the process. |

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