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各位師兄師姐 能不能幫我看看改改摘要 生物類 謝謝 金幣只有這么多了
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可以不用非要按照中文寫的 關(guān)鍵是英文摘要的通順 和語法之類的 謝謝 中文：不同性別家蠶34℃高溫處理后的基因表達(dá)差異分析 為了研究家蠶在34℃環(huán)境下耐受高溫的分子作用機(jī)制,我們構(gòu)建了以抗高溫家蠶7532品種5齡幼蟲高溫處理前后中腸,絲腺和脂肪體（混合）組織為材料的四個SGAE文庫(常溫（26℃）雌,雄，高溫（34℃）雌,雄)。結(jié)果在獲得的4個SAGE文庫中Distinct Tag number為45972,56200,49467,46819，其中能夠比對到基因的分別占到41.10%,35.00%,35.98%,33.16%，獲得了豐富的數(shù)據(jù)。高溫處理后雌,雄家蠶的基因表達(dá)差異較大，雌蠶高溫后有193個基因表現(xiàn)上調(diào)，507個基因下調(diào)，雄蠶高溫后上調(diào),下調(diào)基因數(shù)分別為516,318，另外有許多基因在高溫后表現(xiàn)出特異表達(dá)或沉默。差異基因的GO分析（Gene ontology）發(fā)現(xiàn)雌,雄蠶高溫處理后其功能分布基本一致，而在synapse part,virion part,metallochaperone,cell killing,immune system process,rhythmic process顯示出顯著差異（p<0.05）。KEGG （Kyoto Encyclopedia of Genes and Genomes ） pathway分析顯示主要的差異途徑及差異基因個數(shù)為Oxidative phosphorylation：16個，Purine metabolism：13個，Pyrimidine metabolism：9個，Ubiquitin mediated proteolysis：9個，Aminoacyl-tRNA biosynthesis：8個，Glycolysis / Gluconeogenesis：8個，Arginine and proline metabolism 8個，Ribosome 8個，雖然pathway路徑分析顯示雌雄差異相同，但是每個途徑中參與的基因種類,基因個數(shù)及其上下調(diào)模式均表現(xiàn)出較大差異。在我們的實驗室中，我們首先關(guān)注結(jié)果表明，高溫導(dǎo)致家蠶基因表達(dá)水平上的明顯差異，而這些差異基因也顯示出了性別差異，這些數(shù)據(jù)可以為研究不同性別家蠶對高溫耐受的分子機(jī)理提供研究基礎(chǔ)。 英文：Analysis of differential gene expression of different genders silkworm in 34℃ high temperature treated To investigate the molecular mechanism of silkworm resistance to high temperature and the difference between the sexes, We used serial analysis of gene expression technology （SAGE） to investigate gene expression profiles in the silkworm tissues including the midgut, silk gland and fat body of the 5th instar larvae between high-temperature treated（34℃，Male and Female）and control larvae（26℃，Male and Female）.The results indicate that the Distinct Tag Numbers of the SAGE libraries are 45 972, 56 200, 49 467, 46 819 respectively, and the rates of mapping to genes are 41.10%, 35.00%, 35.98%, 33.16% in the four libraries accordingly. There are large differences between the gene expression of male and female Bombyx mori after heat treatment.193 genes of female Bombyx mori are up-regulated and 507 genes are down-regulated, the male are 516 and 318 respectively. Additionally, plenty of genes are expressed specifically or being silenced after heat treatment. The gene ontology (GO) analysis of differential genes indicated that the functional distributions of male and female after heat treatment are roughly identical, however, significant differences(p<0.05)are showed in synapse part, virion part, metallochaperone, cell killing, immune system process and rhythmic process. The KEGG(Kyoto Encyclopedia of Genes and Genomes)Pathway Analysis reveals the major distinct ways and the number of distinct genes respectively are: Oxidative phosphorylation:16, Purine metabolism:13, Pyrimidine metabolism:9, Ubiquitin mediated proteolysis:9, Aminoacyl-tRNA biosynthesis:8, Glycolysis/Gluconeogenesis: 8, Arginine and proline metabolism: 8, Ribosome: 8. Although the distinctions of male and female are shown to be similar in the pathway analysis, the types, numbers and up/down-regulated models of genes in every different pathway vary considerably. The results show that high temperature has caused significant differences in generic expression levels of bombyx mori , and those distinct genes reveals gender differences. These data can offer research foundation for studying the molecular mechanisms of temperature-resistance of bombyx mori with different sex. [ Last edited by 591508522 on 2013-5-14 at 21:51 ] |
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