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zongsheng09木蟲 (正式寫手)
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[求助]
求解審稿專家有關(guān)文章中的Western blots意見
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小蟲投了一篇Journal of Molecular Medicine,文章修回 其中一位審稿專家的一條意見是如下: “At a technical level, the authors need to show specific binding of antibodies to proteins in Western blots (for Nrf2, Orai1, Stim1) as commercial antibodies are frequently non-specific. This can be done, for instance, by RNAi. List companies antibodies were purchased from and clone numbers. Show entire Western blots (at least in Supplemental Figures), not just isolated bands. It is impossible to evaluate if these bands are specific for the proteins they are supposed to represent.” 前面的意思我能理解,就是要求給出詳細(xì)的抗體廠家和克隆類型,但是接下來的要求在Supplemental Figures增加“entire Western blots”,小蟲實(shí)在不理解,求各位蟲友給出寶貴意見! Ps:我的WB圖片都是,暗室曝光后的膠片,掃描后剪切的成幾個(gè)一橫行的條帶,與SCI文章中常見圖片無異 |
木蟲 (正式寫手)
木蟲 (職業(yè)作家)
我不再應(yīng)助!

木蟲 (職業(yè)作家)
我不再應(yīng)助!

木蟲 (正式寫手)
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