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[求助]
醫(yī)學(xué)翻譯,中譯英,求大神指導(dǎo)。
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Proline dehydrogenase gene is located at chromosome 22q11 deletion region ,and it is considered as the candidate genes of Schizophrenia. 脯氨酸脫氫酶 ( proline dehydrogenase,PRODH)基因位于染色體 22q11 缺失區(qū),被認(rèn)為是精神分裂癥的位置候選基因。 Therefore, to explore the association of PRODH - 1945 ( T/C) polymorphism with schizophrenia in Zhuang and Han ethnic groups in Guangxi Zhuang Autonomous Region ( GZAR),of 282 patients with schizophrenia and 282 healthy controls were genotyped for PRODH - 1945 ( T / C) polymorphism by quantitative real - timePCR using TaqMan - MGB probe technology. 為此,本研究對 282 例壯族、漢族精神分裂癥患者和 282 例壯族、漢族健康對照者,采用 TaqMan MGB 探針等位基因分型技術(shù)對 PRODH - 1945 ( T/C) 多態(tài)性 ( 即 rs372055) 進(jìn)行基因分析,探討該位點與廣西壯族、漢族精神分裂癥易感性的關(guān)聯(lián)性。 1.1.1case group including Zhuang and Han ethnic groups , the inclusion and exclusion criteria of the identified studies as follows 1) the inclusion criteria: ①To keep the homogeneity of the cases , the diagnoses of schizophrenia according to ICD - 10 criteria were confirmed by 2 senior psychiatric specialists,who were strictly trained , using the consistency check of diagnostic test ; 1. 1. 1 病例組 包括壯族病例組和漢族病例組,納入與排除標(biāo)準(zhǔn)如下: ( 1) 納入標(biāo)準(zhǔn): ①所有患者經(jīng)至少兩名副主任醫(yī)師以上職稱的精神科?漆t(yī)師根據(jù)國際疾病及相關(guān)健康問題分類第 10 版 ( ICD -10) 精神與行為障礙分類臨床描述與診斷要點確診為精神分裂癥患者,并對實施診斷的醫(yī)師經(jīng)過嚴(yán)格培訓(xùn),進(jìn)行診斷一致性檢驗,保證入組對象的同質(zhì)性; 因嚴(yán)重興奮、沖動不配合者,或者有嚴(yán)重自殺、自傷傾向者。(中譯英) 上述 564 例受試者之間均無上代族間通婚史,也無親緣關(guān)系。。(中譯英) ②patient self (or their immediate families) reported patients in three generation tof family descended from the zhuang population.③patient self (or their immediate families) reported patients is Guangxi native ②患者自我 ( 或其直系家屬) 報告患者三代內(nèi)均為本民族血緣; ③患者自我 ( 或其直系家屬) 報告患者籍貫為廣西人。 1.2 genotype we put the participants’peripheral venous blood(5ml) samples into Vacuum Blood Collection Tube with EDTA, and mixed and Stored in 4℃,then extracted Genomic DNA (samples) within 3 days 1. 2 基因分型 采集每例受試者的外周靜脈血 5 ml,置于含有 EDTA 抗凝劑的真空采血管內(nèi),混勻,4 ℃ 保存,并于 3 d內(nèi)提取基因組 DNA。 According to the product directions,Genomic DNA were strictly operate to extract the blood by using blood Genomic DNA kit produced by TianGen technology(Beijing) Company Limited〔TIANamp Blood DNA Kit ,Catalog Number DP318DNA〕 采用天根科技 ( 北京) 有限公司生產(chǎn)的血液基因組 DNA 提取試劑盒 〔TIANamp Blood DNA Kit ( 離心柱形) ,目錄號 DP318DNA〕,血液基因組 DNA 的提取嚴(yán)格按照產(chǎn)品說明書操作。 |
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