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北京石油化工學(xué)院2026年研究生招生接收調(diào)劑公告

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847037274

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In this paper, a direct and simple detection method based on surface-enhanced Raman scattering (SERS) named “heat-induced SERS sensing method” is proposed for rapid determination of glutathione in aqueous solutions. It was found that highly enhanced SERS spectra of glutathione can be obtained if the silver colloids adsorbed with the analyte were heated up before the SERS measurement. Besides, it was revealed that silver particles with a size of 60 nm are suitable for this study and that the SERS intensity is also influenced by the dropped sample volume, drying temperature, buffer concentration, and pH of the solution. It is noted that the thiol group of glutathione has a particularly strong interaction with a silver surface compared with other small biological molecules without a thiol group, validating this method to detect glutathione selectively. Under the optimal conditions, the detection of glutathione can be finished within 5 min, and the detection limit of ca. 50 nM can be reached, which is much better than the reported detection limit of glutathione (1 μM) by SERS. The enhancement factor of the proposed heat-induced SERS sensing method for the detection of glutathione is about 7.5 × 106. The proposed method holds a specific selectivity toward glutathione, facilitating its rapid detection in practical applications.

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1樓 2013-06-16 14:32:36

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luodiheng

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847037274(phu_grassman代發(fā)): 金幣+5, 翻譯EPI+1, thanks，SERS是表面增強(qiáng)拉曼 2013-11-07 10:19:02

在此文中，一個(gè)基于表面增效Raman掃描（SERS）叫做"熱誘導(dǎo)SERS感應(yīng)手段"的直接而簡(jiǎn)單的檢測(cè)手段被提出來用于快速檢測(cè)水溶液中的谷胱甘肽。如果吸附了被檢物的含銀膠質(zhì)物在SERS檢測(cè)前加熱的話，可以得到極大提高的谷胱甘肽SERS光譜。另外還發(fā)現(xiàn)，60nm的銀粒子是最適合的，另外SERS強(qiáng)度也受樣本體積，干燥溫度，緩沖液濃度和溶液PH值的影響。另外觀察到的是，與其他沒有ＳＨ的生物小分子相比，谷胱甘肽的ＳＨ與銀表面有著特別強(qiáng)的相互作用，使得這種檢測(cè)手段具有谷胱甘肽選擇性。在最優(yōu)條件下，谷胱甘肽檢測(cè)可以在５分鐘內(nèi)完成，檢測(cè)極限達(dá)50 nM，這比報(bào)道的谷胱甘肽SERS檢測(cè)極限1uM好得多。熱誘導(dǎo)SERS感應(yīng)手段相對(duì)于普通的谷胱甘肽檢測(cè)靈敏度提高因子大約能達(dá)7.5X106。由于此檢測(cè)手段對(duì)于谷胱甘肽的特異選擇性，使得他在實(shí)際應(yīng)用中成為快速檢測(cè)谷胱甘肽一種手段。

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因吹斯聽

2樓2013-06-16 14:56:48

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