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youlizujuan新蟲 (小有名氣)
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[求助]
本人英語不好，需要下面的英文資料，求大神翻譯兩段文獻(xiàn)。感激不盡�。�
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Identification of degradation intermediates. High-performance liquid chromatography (HPLC) analyses were done as previously described, except an Alltech Alltima Amino column (250 mm by 4.6 mm, 5- mparticles) was used with an isocratic mobile phase consisting of 78% acetonitrile and 22% 5 mM phosphate buffer (pH 6.7) at a flow rate of 0.375 ml/min. Retention times for melamine, ammeline, ammelide, and cyanuric acid standards monitored at 224 nm were 14.8 to 17.0, 32.4 to 35.6, 27.0 to 29.0, and 14.2 to 17.2 min, respectively. Melamine and cyanuric acid had overlapping retention times but were differentiated by their UV absorbance peaks at 202 nm and 214 nm, respectively. Screening for melamine deaminase genes and sequencing of trzA flanking regions. The presence oftrzA or triA in Rhodococcus sp. strain Mel was determined by PCR using the primers trzA_309F and trzA_787R (see Table S1 in the supplemental material), designed from the published sequences from strain NRRL B-15444R, and the atzA primers, which were shown to amplify an internal fragment from triA. Primers used to sequence the remainder oftrzA and flanking regions corresponding to the published trzA contig are shown in Table S1. Genome sequencing and analyses.Total genomic DNA from Rhodococcus sp. strain Mel was sequenced using a Roche GS 454 FLX system and standard LR 70 chemistry at the University of Minnesota BMGC. Sequences were assembled, and 423 of 440 contigs were submitted to Genoscope for annotation and synteny analysis using the MicroScope and Magnifying Genomes (MaGe) interface platforms. Magnifying Genomes (MaGe) interface platforms. |
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