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songsong000鐵蟲 (初入文壇)
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[求助]
流式檢測
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| 在流式檢測過程之前的細(xì)胞的提取部分,都有哪些因素會(huì)影響抗體與抗原的結(jié)核性,哪些步驟可以讓結(jié)果更好些?在檢測過程中,流式儀的操作,那些需要注意?? |

木蟲 (正式寫手)
| 細(xì)胞提取部分?指的是細(xì)胞裂解液么?影響抗體跟抗原結(jié)合有很多因素,比如說你的細(xì)胞是活細(xì)胞,還是固定過的?你的細(xì)胞是培養(yǎng)基里重懸還是在緩沖液里?緩沖液里重懸細(xì)胞是否對(duì)細(xì)胞造成了影響?至于流式的操作,這個(gè)真的很難說的,結(jié)果的好壞跟你設(shè)門以及對(duì)照的選擇有很重要的關(guān)系的哦~還有選用的熒光素在激光器的激發(fā)波長下發(fā)光效率是否高?這都是實(shí)驗(yàn)前要詳細(xì)查詢和咨詢的呢~~ |
| It may depend on what kind of cells you are using, if you are using cell line, you may not meet many issues. If you are using cells from tissues. Be sure to get some pure cell population by using some isolation kit or reagents. During staining, be sure to optimize your primary antibody staining condition such ad dilution and time. Wash appropriate times. During flow, dilute your sample to optimal concentration, and choose optimal flow speed. |

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