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huojinlong8610金蟲(chóng) (小有名氣)
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要求潤(rùn)色英語(yǔ) 【摘要】目的 獲得版納微型豬近交系生長(zhǎng)激素受體（Growth hormone receptor，GHR）基因序列，通過(guò)生物信息學(xué)分析預(yù)測(cè)GHR功能并進(jìn)行GHR mRNA多組織表達(dá)譜分析。方法 以版納微型豬近交系的肝臟組織為材料提取RNA，RT-PCR方法擴(kuò)增GHR基因編碼區(qū)序列，將序列連接至pMD18-T載體進(jìn)行克隆、測(cè)序和生物信息學(xué)分析；半定量方法檢測(cè)GHR mRNA在BMI不同組織中表達(dá)量的差異。結(jié)果 成功克隆了BMI GHR 編碼區(qū)序列，提交GenBank獲得登錄號(hào)KC999114。該基因CDS長(zhǎng)1917 bp，編碼638個(gè)氨基酸。生物信息學(xué)分析表明，與長(zhǎng)白豬的GHR序列相比BMI存在4處氨基酸替換，分別為p. E381D，p. A409S，p. L556V和p. A580G，均發(fā)生在GHBP區(qū)。GHR基因多組織表達(dá)譜分析顯示：GHR mRNA幾乎在各組織中均有表達(dá)，其中在神經(jīng)纖維、心、肝、脾、肺、小腸、卵巢和肌肉中表達(dá)量都很高，在大腦、腎、胃和胰中的表達(dá)量較低。結(jié)論 GHR的氨基酸替換可能會(huì)影響GHR與GH的結(jié)合，導(dǎo)致其體型矮小。 [Abstract] Objective To get the BMI GHR gene sequence, predict its function by bioinformatics analysis and obtain the GHR mRNA tissues transcription profile. Methods BMI GHR cDNA sequence was cloned from liver RNA by RT-PCR. Inserted the product into pMD18-T vector for clone, sequencing and bioinformatics analysis. Conducted semi-quantitative RT-PCR to determine its expression in different tissues. Results The GHR cDNA sequence was cloned and got the GenBank Accession No. KC999114. The encoding sequence was 1917 bp and encoded a protein of 638 amino acids. Bioinformatics analysis showed that there were four amino acids substitutions were found in the GHR binding protein superfamilies between BMI and Landrace pig GHR protein. The substitutions were p. E381D, p. A409S, p. L556V and p. A580G. GHR mRNA tissues expression analysis revealed that GHR mRNA was expressed in almost all tissues. It was most highly expressed in the nerve fiber, heart, liver, spleen, lung, small intestine, ovary and muscle while weakly expressed in the brain, kidney, stomach and pancreas. Conclusions The BMI GHR substitutions maybe weaken the interaction of GHR and GH which can lead to BMI dwarfism. |
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biomed_fanyi 
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huojinlong8610: 金幣+20, 翻譯EPI+1, ★有幫助 2013-07-29 17:46:31
huojinlong8610: 金幣+20, 翻譯EPI+1, ★有幫助 2013-07-29 17:46:31
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本帖內(nèi)容被屏蔽 |
2樓2013-07-29 08:31:08
lemon_time
金蟲(chóng) (小有名氣)
- 翻譯EPI: 1
- 應(yīng)助: 0 (幼兒園)
- 金幣: 784.5
- 帖子: 55
- 在線: 13.8小時(shí)
- 蟲(chóng)號(hào): 1337133
- 注冊(cè): 2011-07-04
- 專業(yè): 動(dòng)物資源與保護(hù)
【答案】應(yīng)助回帖
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huojinlong8610: 金幣+80, ★★★★★最佳答案 2013-07-29 17:46:40
huojinlong8610: 金幣+80, ★★★★★最佳答案 2013-07-29 17:46:40
| [Abstract] Objective To get the sequence of BMI GHR gene, predict its function by bioinformatics analysis and investigate its mRNA expression profile in different tissues. Methods BMI GHR cDNA sequence was cloned from liver RNA by RT-PCR. Then the products were inserted into pMD 18-T vector for cloning, sequencing and bioinformatics analysis. It was to determine GHR mRNA expression in different tissues by conducting semi-quantitative RT-PCR. Results The GHR cDNA sequence was cloned and the the GenBank Accession No. was KC999114. The encoding sequence was 1917 bp and encoded a protein of 638 amino acids. The bioinformatics analysis result showed that there were four amino acids substitutions at the GHBP superfamilies of GHR protein comparing BMI with Landrace. The substitutions were p. E381D, p. A409S, p. L556V and p. A580G. Tissues expression analysis revealed that GHR mRNA was expressed in almost all tissues. It was most highly expressed in the nerve fiber, heart, liver, spleen, lung, small intestine, ovary and muscle while weakly expressed in the brain, kidney, stomach and pancreas. Conclusions The GHR substitutions may affect the interaction of GHR and GH which can infulence the BMI growth and development. |
3樓2013-07-29 17:44:51
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