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lairongpeng銀蟲 (正式寫手)
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[求助]
求教經(jīng)?从⑽奈墨I的大神,這些是啥意思呢?
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pyrG89; wA3; pyroA4 [pyr4 + copA::gfp] (pyrG89; pyroA4; nkuA::argB) pAL12-Lifeact [Ptef-1::Lifeact-TagRFP-T::nat1] sepA::sGFPpyrG89;argB2;ΔbemA::argB;sE15,fwA1(ΔnkuB::pyrG;nkuA;argB;uaY9) |
至尊木蟲 (著名寫手)
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http://www.fgsc.net/scripts/Stra ... age.asp?OrgID=11562 Lesion: pyrG89 pyrimidine req. orotidine MP decarboxylase Enzyme Name: orotidine 5'-monophosphate decarboxylase Lesion Information for Marker: Linkage Group of pyrG89: IL Marker description or requirements: Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors Reference for: pyrG89: 10167. de Souza et al. 1998. Curr. Genet. 33,60-69 Reference for: pyrG89: 10472. Oakley, B. R. et al. 1987. Gene 61;385-399 Reference for: pyrG89: 10486. Palmer, L. M. & D. J. Cove 1975. Mol. Gen. Genet. 138,243-255. Lesion: pyroA4 pyridoxine req. Lesion Information for Marker: Linkage Group of pyroA4: IVR Marker description or requirements: Responds to pyridoxine Reference for: pyroA4: 10332. Kafer, E. 1958. Adv. Genet. , 9,105. Lesion: nkuA::argB Marker description or requirements: Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs. |

至尊木蟲 (著名寫手)
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http://www.nature.com/nprot/jour ... ot.2007.112_T2.html Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored |

銀蟲 (正式寫手)
至尊木蟲 (著名寫手)

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