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[求助]
請問melting curves的圖怎么做
最近投文章,審稿人問了個問題,說我melting curves的斜率太大,我找了很多文獻終于發(fā)現(xiàn)問題所在,但不知道怎么改。
我做的圖大約像這張圖A的樣子,我估計審稿人看的都是圖B的樣子。這篇文章中圖B是圖A標準化后的結果。請問這兩張圖怎么互轉,所謂的標準化是什么意思。
圖例說明:Figure 3.
Genotyping of two adjacent single-nucleotide polymorphisms (HbS and HbC) within a 110-bp β-globin fragment with use of high-resolution amplicon melting curve analysis.
The original high-resolution melting curves (A) include two different individuals for each of the six different genotypes, each individual run in duplicate. (B), the same curves as in panel A after normalization. (C), normalized curves after temperature-shifting of each curve to overlay them on one of the AA curves between 5% and 10% fluorescence. (D), fluorescence difference curves obtained by subtracting each curve in panel C from one wild-type (AA) curve (for clarity, the other AA samples are not shown). Because of the shift in temperature of the curves in panels C and D, the temperature axis no longer reflects absolute temperatures but rather reflects temperature differences relative to the superimposed segments of the curves.
![請問melting curves的圖怎么做]()
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