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haolw411金蟲(chóng) (小有名氣)
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[求助]
英文文獻(xiàn)翻譯
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學(xué)制劑的，該文獻(xiàn)很是看不懂，求大神幫忙翻譯文獻(xiàn)中的以下兩段： We have previously reported the critical roles of Rac2 in a wide variety of primary hematopoietic cells, including the regulation of adhesion,migration, oxidase activity, and gene expression.These same functions have been attributed to Rac1 in nonhematopoietic cells in whichRac2 is not expressed. However, the roles of Rac1 compared with the roles of Rac2 in blood cells, which express both GTPases, remain to be elucidated. Because homozygous Rac1-deficient mice die at E8 (embryonic day 8) in utero , we generated mice with a conditional Racl(flox)allele (Rac1 flox/flax)(fig S1).These were compared with mice that were homozygous for both the Racl flox allele and a Rac2-null allele(Rac1flox/flox;Rac2–/–). Floxed Rac1 sequences were deleted by means of two methods, and the deletion of Rac1 sequences was confirmed with polymerase chain reactions (PCRs) and immuno-blots (figs. S2 and S3). |
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