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xxls1990鐵蟲 (初入文壇)
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[求助]
Vibrio parahemolyticus 副溶血性弧菌的16rsDNA
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| 怎么有數(shù)據(jù)庫(kù)中找副溶血弧菌的16srDNA序列,又怎樣根據(jù)所有菌的16srDNA的保守序列設(shè)計(jì)引物???急,非常感謝 |


鐵蟲 (初入文壇)

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建議你用通用引物。因?yàn)槟愕哪康木褪菧y(cè)序,不是設(shè)計(jì)鑒定引物吧? Unfortunately, 16S rRNA sequences revealed 99.7% homology between V. parahaemolyticus and V. alginolyticus, and thus 16S rRNA was unable to differentiate between V. parahaemolyticus and other vibrios (18). 18. Ruimy R, Breittmayer V, Elbaze P, Lafay B, Boussemart O, Gauthier M, Christen R. Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences. Int J Syst Bacteriol. 1994;44:416–426. |

鐵蟲 (初入文壇)

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