 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 4 | 1/1 | 返回列表 |
| 查看: 744 | 回復: 3 | ||
[求助]
有誰知道精氨酸在蛋白正確折疊組裝時起到的作用？？？
|
如題~很多蛋白的組裝液中都含有精氨酸，有誰知道精氨酸在蛋白正確折疊組裝時起到的作用？？？要分子層面的，是因為精氨酸的長側鏈能起到什么緩沖扶持的作用么？我也就是猜測，請各位大神們幫我解答下啊，不要宏觀的作用，想要分子層面的機理~~~�。。≈x了！  |
回復此樓» 猜你喜歡
不合理蛙科研實驗中的趣事：實驗器材的 “烏龍”
已經有0人回復
-
不合理蛙科研實驗中的趣事：和實驗材料的 “斗智斗勇”
已經有0人回復
-
化學工程及工業(yè)化學論文潤色/翻譯怎么收費?
已經有194人回復
-
蛋白質檢測：精準分析，解鎖生物分子的密碼
已經有0人回復
-
不合理蛙科研實驗之小鼠實驗：嚴謹設計，解析生命機制的重要載體
已經有0人回復
-
不合理蛙科研實驗之重金屬檢測：精準篩查，守護健康與環(huán)境的防線
已經有0人回復
-
不合理蛙科研實驗之“蛙測重金屬我背鍋三千”
已經有0人回復
-
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI”
已經有0人回復
-
納米粒度分析
已經有3人回復
-
林科院林化所招收材料與化工專業(yè)碩士，坐標南京
已經有0人回復
» 本主題相關商家推薦: (我也要在這里推廣)
» 本主題相關價值貼推薦，對您同樣有幫助:
- 帶有精氨酸及尿素的變性蛋白如何純化？
已經有3人回復
沒人么？舉例說明也好呀
2樓2013-10-27 23:15:48
3樓2013-10-28 12:08:43
xl_dut
木蟲 (正式寫手)
- 應助: 13 (小學生)
- 金幣: 1384.8
- 帖子: 404
- 在線: 156.4小時
- 蟲號: 2048813
- 注冊: 2012-10-08
- 性別: GG
- 專業(yè): 微生物遺傳育種學
【答案】應助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感謝參與，應助指數 +1
莓林塔(kx444555代發(fā)): 金幣+3, 鼓勵交流 2013-10-28 15:04:58
莓林塔: 金幣+17, ★★★★★最佳答案, 謝謝 ~ 2013-10-29 17:05:50
感謝參與，應助指數 +1
莓林塔(kx444555代發(fā)): 金幣+3, 鼓勵交流 2013-10-28 15:04:58
莓林塔: 金幣+17, ★★★★★最佳答案, 謝謝 ~ 2013-10-29 17:05:50
|
建議樓主看一下這篇文章！ Biotechnol Prog. 2004 Sep-Oct;20(5):1301-8. Role of arginine in protein refolding, solubilization, and purification. Tsumoto K, Umetsu M, Kumagai I, Ejima D, Philo JS, Arakawa T. Source Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai 980-8579, Japan. tsumoto@mail.tains.tohoku.ac.jp Abstract Recombinant proteins are often expressed in the form of insoluble inclusion bodies in bacteria. To facilitate refolding of recombinant proteins obtained from inclusion bodies, 0.1 to 1 M arginine is customarily included in solvents used for refolding the proteins by dialysis or dilution. In addition, arginine at higher concentrations, e.g., 0.5-2 M, can be used to extract active, folded proteins from insoluble pellets obtained after lysing Escherichia coli cells. Moreover, arginine increases the yield of proteins secreted to the periplasm, enhances elution of antibodies from Protein-A columns, and stabilizes proteins during storage. All these arginine effects are apparently due to suppression of protein aggregation. Little is known, however, about the mechanism. Various effects of solvent additives on proteins have been attributed to their preferential interaction with the protein, effects on surface tension, or effects on amino acid solubility. The suppression of protein aggregation by arginine cannot be readily explained by either surface tension effects or preferential interactions. In this review we show that interactions between the guanidinium group of arginine and tryptophan side chains may be responsible for suppression of protein aggregation by arginine. |
4樓2013-10-28 13:14:38
相關版塊跳轉
我要訂閱樓主 莓林塔 的主題更新
| 4 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 323求調劑 +6 | 洼小桶 2026-03-18 | 6/300 |
|
|---|---|---|---|---|
|
|
[考研] 311求調劑 +6 | 冬十三 2026-03-18 | 6/300 |
|
|
[考研] 求調劑 +6 | 十三加油 2026-03-21 | 6/300 |
|
|
|
[考研] 289求調劑 +7 | 懷瑾握瑜l 2026-03-20 | 7/350 |
|
|
|
[考研] 275求調劑 +6 | shansx 2026-03-22 | 8/400 |
|
|
|
[考研] 能源材料化學課題組招收碩士研究生8-10名 +5 | 脫穎而出 2026-03-16 | 17/850 |
|
|
|
[考研] 生物學調劑 +5 | Surekei 2026-03-21 | 5/250 |
|
|
|
[考研] 一志愿北京化工大學070300 學碩336求調劑 +5 | vv迷 2026-03-21 | 8/400 |
|
|
|
[考研] 初試 317 +7 | 半拉月丙 2026-03-20 | 7/350 |
|
|
|
[考研] 化學調劑 +5 | yzysaa 2026-03-21 | 5/250 |
|
|
|
[考研] 材料與化工（0856）304求 B區(qū) 調劑 +3 | 邱gl 2026-03-21 | 3/150 |
|
|
|
[考研] 一志愿天津大學化學工藝專業(yè)（081702）315分求調劑 +12 | yangfz 2026-03-17 | 12/600 |
|
|
|
[考研] 一志愿南京理工大學085701資源與環(huán)境302分求調劑 +4 | 葵梓衛(wèi)隊 2026-03-18 | 6/300 |
|
|
|
[考研]
 295復試調劑
+8
|
簡木ChuFront 2026-03-19 | 8/400 |
|
|
|
[考研] 0817 化學工程 299分求調劑 有科研經歷 有二區(qū)文章 +22 | rare12345 2026-03-18 | 22/1100 |
|
|
|
[考研] 0856調劑，是學校就去 +8 | sllhht 2026-03-19 | 9/450 |
|
|
|
[考研] 招收調劑碩士 +4 | lidianxing 2026-03-19 | 12/600 |
|
|
|
[考研] 286求調劑 +6 | lemonzzn 2026-03-16 | 10/500 |
|
|
|
[考研] 328求調劑，英語六級551，有科研經歷 +4 | 生物工程調劑 2026-03-16 | 12/600 |
|
|
|
[考研] 333求調劑 +3 | 文思客 2026-03-16 | 7/350 |
|