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| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | ||
bartob123321銅蟲 (小有名氣)
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[求助]
求助翻譯一段英文——關(guān)于小腸移植排斥藥物
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| CHO-K1 cells stably expressing hS1P1 or hS1P3 were plated at 2×104 cells/well in a 96-well plate and incubated for 2 days at 37 °C in 5% CO2/95% air. The cells were loaded with 5 μM Fura2-AM and 20 mM HEPES (pH 7.4) in Ham's F12 medium at 37 °C for 1 h.After loading, the cells were washed with Hanks' solution containing 20 mM HEPES (pH 7.4) and stimulated with S1P,FTY-P,andW-061.Fluorescence intensity was measured by the ratio of emission fluorescence at 500 nm to excitation at 340 and 380 nm using a Fluorescence Drug Screening System. |
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xux730
鐵桿木蟲 (著名寫手)
- 翻譯EPI: 86
- 應(yīng)助: 1719 (講師)
- 金幣: 10740.4
- 散金: 50
- 紅花: 43
- 帖子: 2947
- 在線: 336.5小時
- 蟲號: 1374299
- 注冊: 2011-08-20
- 專業(yè): 細胞周期與調(diào)控
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
愛與雨下: 金幣+1 2013-11-01 06:28:20
bartob123321: 金幣+40, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2013-11-01 20:28:05
愛與雨下: 金幣+1 2013-11-01 06:28:20
bartob123321: 金幣+40, 翻譯EPI+1, ★★★★★最佳答案, 謝謝 2013-11-01 20:28:05
| 將穩(wěn)定表達hS1P1或hS1P3的CHO-K1細胞接種在96孔板里（每孔2×104個細胞），在5％CO2培養(yǎng)箱中在37℃下培養(yǎng)2天。在Ham氏F12培養(yǎng)基中加入Fura2-AM和HEPES（pH7.4）使其終濃度分別為5 μM和20mM，在37℃下繼續(xù)培養(yǎng)1小時。隨后，用含有20mM HEPES（pH7.4）的漢斯溶液洗細胞，用S1P、FTY-P和W061刺激細胞。用熒光藥物篩選系統(tǒng)測定熒光強度（在500nm發(fā)射的熒光與在340和380nm處激發(fā)的熒光的比值）。 |
2樓2013-10-31 21:45:51
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