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谷胱甘肽單乙酯合成（GSH-OEt）方法 已有1人參與
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GSH-OEt購(gòu)買的話，SIGMA是600多元25mg,1860元500mg,稍貴。 以下為其簡(jiǎn)便合成方法。 原來看到有人在求助，GSH-OEt哪里買？或者說怎么合成。 合成過程如下，和大家分享。 A freshly prepared solution of hydrogen chloride (16 g, 0.45 mol) in 250 ml of anhydrous ethanol was cooled to 20°C in an ice bath, and 20 g (65 mmol) of glutathione was added and dissolved by gentle shaking. The mixture was allowed to stand at 20°C for 2.5 h, during which time the progress of the reaction was followed by thin-layer chromatography (see Materials and Methods). The solution was then cooled to 0°C and 1.8 liter of ice-cold, dry diethyl ether was added. The precipitate which formed was allowed to settle for l- 3 h at 0°C. The ether was decanted and the precipitate was washed twice with 500 ml of ice-cold ether. The product, after drying in vacua over CaClz and KOH, was obtained in 98% yield. The content of glutathione monoethyl ester found on amino acid analysis (see Materials and Methods) varied from 90 to 98%; the other components were glutathione and glutathione diethyl ester. The preparations obtained typically contained O-5% glutathione. 文獻(xiàn) Glutathione monoethyl ester: Preparation, uptake by tissues, and conversion to glutathione Archives of biochemistry and biophysics 1985 Jun 239(2):538-48. |
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