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張寧強(qiáng)08木蟲(chóng) (著名寫(xiě)手)
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[求助]
材料緩釋 已有1人參與
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| 我現(xiàn)在想做緩釋,我們實(shí)驗(yàn)室條件只能用透析袋進(jìn)行緩釋,小弟請(qǐng)教一般透析袋緩釋怎么去做呢? |

木蟲(chóng) (著名寫(xiě)手)

榮譽(yù)版主 (文壇精英)
孩兒她爹
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1、根據(jù)藥物分子量確定透析袋品種 2、根據(jù)藥物溶解性,確定釋放液組分及體積 3、最后將較高濃度的載藥粒子放入透析袋,然后移入較大體積釋放液中透析,按時(shí)測(cè)定透析液中濃度即可 這是一篇用透析法測(cè)定阿霉素釋放的描述,你可以借鑒一下。 Freeze-dried DOX-loaded micelles (1.0 mg) were suspended in 2 mL PBS, and the suspension was then dialyzed against 30 mL PBS at 37°C under stirring at 90 ± 5 rpm. At desired time intervals, 5 mL of the medium were taken out and replenished with an equal volume of fresh media. The amount of DOX released was measured by HPLC equipped with a C18 column (L6-P6, Purkinje General, Beijing, China). The mobile phase was a mixture of CH3OH : CH3CN : H2O : H3PO4 = 12 : 100 : 112 : 0.15 (volume ratio), the flow rate was 1.0 mL/minutes at 25°C, and the wavelength was set at 480 nm. |

木蟲(chóng) (著名寫(xiě)手)

榮譽(yù)版主 (文壇精英)
孩兒她爹

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