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lennon_張銅蟲(chóng) (小有名氣)
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[求助]
求助翻譯，關(guān)于LIVE/DEAD assay這個(gè)實(shí)驗(yàn)方法的 急用~~~
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篇文獻(xiàn)用到LIVE/DEAD assay這個(gè)實(shí)驗(yàn)方法，其中的步驟有一段不太理解，求大神幫助��！ Cells were detached from the substrate with Trypsin and counted prior to centrifugation. Prior to hydrogel crosslinking, 25,000 cells were suspended in 100 lL aliquots of each hydrogel precursor solution (n ¼ 3). Each aliquot was transferred to wells in a 96-well plate after which the solutions were allowed to crosslink or crosslinking was initiated by the appropriate method. After gelation, 100 lL of Keratinocyte SFM was added to each well. After 24 h in culture, media was removed and 100 lL of 4 mM calcein-AM and 4 mM ethidium homodimer-1 assay solution was added to each well. |
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