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zmfzj至尊木蟲 (著名寫手)
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[求助]
Transcriptome of Vector, and the Molecular Interactions of virus Transmission
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Transcriptome of the Plant Virus Vector Graminella nigrifrons, and the Molecular Interactions of Maize fine streak virus Transmission Abstract Background: Leafhoppers (Hemiptera: Cicadellidae) are plant-phloem feeders that are known for their ability to vector plant pathogens. The black-faced leafhopper (Graminella nigrifrons) has been identified as the only known vector for the Maize fine streak virus (MFSV), an emerging plant pathogen in the Rhabdoviridae. Within G. nigrifrons populations, individuals can be experimentally separated into three classes based on their capacity for viral transmission: transmitters, acquirers and non-acquirers. Understanding the molecular interactions between vector and virus can reveal important insights in virus immune defense and vector transmission. Results: RNA sequencing (RNA-Seq) was performed to characterize the transcriptome of G. nigrifrons. A total of 38,240 ESTs of a minimum 100 bp were generated from two separate cDNA libraries consisting of virus transmitters and acquirers. More than 60% of known D. melanogaster, A. gambiae, T. castaneum immune response genes mapped to our G. nigrifrons EST database. Real time quantitative PCR (RT-qPCR) showed significant down-regulation of three genes for peptidoglycan recognition proteins (PGRP – SB1, SD, and LC) in G. nigrifrons transmitters versus control leafhoppers. Conclusions: Our study is the first to characterize the transcriptome of a leafhopper vector species. Significant sequence similarity in immune defense genes existed between G. nigrifrons and other well characterized insects. The down-regulation of PGRPs in MFSV transmitters suggested a possible role in rhabdovirus transmission. The results provide a framework for future studies aimed at elucidating the molecular mechanisms of plant virus vector competence. |
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zmfzj: 金幣+55, 翻譯EPI+1 2014-02-10 07:26:35
zmfzj: 金幣+55, 翻譯EPI+1 2014-02-10 07:26:35
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黑面（臉）葉蟬植物病毒載體轉(zhuǎn)錄（物）組及其在玉米細條紋病毒傳播時與玉米細條紋病毒分子的相互作用 摘要 背景：葉蟬（半翅目：葉蟬科）被稱為植物韌皮部饋線，因其能攜帶植物病原體而得名。黑臉葉蟬（Graminella nigrifrons）已被確定為（炮彈病毒）彈狀病毒科唯一已知的玉米細條紋病毒（MFSV）載體，一個新興的植物病原體。黑臉葉蟬種群個體可通過實驗基于他們對病毒傳播能力分為三類：傳染源，acquirers and non-acquirers。了解病毒載體和病毒分子間的相互作用可以洞察病毒免疫防御和病毒載體傳播的重要關(guān)鍵。 結(jié)果：RNA測序（RNA的序號）表征黑臉葉蟬轉(zhuǎn)錄組�？偣伯a(chǎn)生38,240個長度超過100 BP的基因表達序列標(biāo)簽（Expressed Sequence Tag ，EST ），可從兩個不同的包含病毒傳染源和 acquirers的cDNA庫比對。已知的黑腹果蠅，岡比亞按蚊，赤擬谷盜免疫反應(yīng)基因 ，60％以上可以映射到黑臉葉蟬基因表達序列標(biāo)簽數(shù)據(jù)庫。實時定量PCR（RT-qPCR的）顯示黑臉葉蟬與對照葉蟬相比，三個與肽聚糖識別蛋白（PGRP - SB1，SD，和LC）有關(guān)的基因顯著下調(diào)。 結(jié)論：本研究第一次表征葉蟬載體物種的轉(zhuǎn)錄組。在黑臉葉蟬與其他已進行很好表征的昆蟲之間免疫防御基因存在顯著序列相似 。MFSV傳染源PGRPs下調(diào)提示可能是彈狀病毒傳播的作用。研究結(jié)果對未來旨在闡明植物病毒載體能力分子機制的研究提供了一個框架。 這是一篇2012PLOS ONE 上的文章，acquirers ， non-acquirers在病毒學(xué)上是指什么東西不太清楚。 |
2樓2014-02-09 23:52:55
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