 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 1763 | 回復(fù): 4 | |||
| 當(dāng)前只顯示滿足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | |||
[交流]
求助酶活測(cè)定方法 PAL、CHS、F3H、DFR、LDOX
|
|||
|
誰(shuí)能提供以下植物中 酶活性測(cè)定方法： PAL、CHS、F3H、DFR、LDOX 謝謝了！ |
回復(fù)此樓» 猜你喜歡
347求調(diào)劑
已經(jīng)有4人回復(fù)
-
一志愿西交288化工專碩求調(diào)劑
已經(jīng)有3人回復(fù)
-
一志愿中國(guó)科學(xué)院大學(xué)265求調(diào)劑
已經(jīng)有4人回復(fù)
-
291求調(diào)劑
已經(jīng)有13人回復(fù)
-
070300化學(xué)求調(diào)劑
已經(jīng)有3人回復(fù)
-
322求調(diào)劑
已經(jīng)有7人回復(fù)
-
0703化學(xué)
已經(jīng)有15人回復(fù)
-
材料學(xué)碩333求調(diào)劑
已經(jīng)有15人回復(fù)
-
313求調(diào)劑
已經(jīng)有3人回復(fù)
-
083000學(xué)碩274求調(diào)劑
已經(jīng)有11人回復(fù)
» 搶金幣啦！回帖就可以得到:
-
資源與環(huán)境專碩
+1/72
-
江西科技師范大學(xué)柔性電子創(chuàng)新研究院OTE-lab招收調(diào)化學(xué)劑研究生
+1/54
-
武漢工程大學(xué)化環(huán)學(xué)院程老師課題組招收化學(xué)類學(xué)碩、化學(xué)工程與環(huán)境工程專碩調(diào)劑
+2/46
-
重慶交通大學(xué)院士團(tuán)隊(duì)招收材料、能源動(dòng)力專業(yè)碩士研究生
+2/40
-
招收2026級(jí)環(huán)境科學(xué)方向（專業(yè)代碼083001）碩士研究生1-2名
+1/36
-
首都師范大學(xué)招收材料與化工專碩調(diào)劑生--中科院半導(dǎo)體所共培
+1/35
-
上海大學(xué) “生物有機(jī)電子材料及器件”團(tuán)隊(duì)碩士、博士研究生招聘
+1/34
-
贛南師范大學(xué)智能制造與未來(lái)能源學(xué)院2026年碩士調(diào)劑信息
+1/29
-
重慶工商大學(xué) 教育部研究中心招收化工/環(huán)境/材料/石油/機(jī)械方向2026年調(diào)劑研究生
+1/11
-
長(zhǎng)江大學(xué)石油工程學(xué)院2026年碩士調(diào)劑招生公告!!!
+1/9
-
【博士招生】天津理工大學(xué)國(guó)家杰青王鐵課題組招收2026年博士研究生
+1/8
-
三峽大學(xué)材料與化工學(xué)院調(diào)劑
+1/7
-
五邑大學(xué)紡織科學(xué)與工程學(xué)院“智能紡織材料與紡織品”研究團(tuán)隊(duì)接收碩士調(diào)劑生
+1/7
-
南京林業(yè)大學(xué)化學(xué)工程學(xué)院柏惺峰課題組誠(chéng)聘科研助理、水杉英才E類/F類青年教師或博后
+1/6
-
材料與化工、環(huán)境科學(xué)專業(yè)調(diào)劑招生
+1/6
-
北京高校副校長(zhǎng)團(tuán)隊(duì)招收機(jī)械類，環(huán)境類學(xué)碩和專碩
+1/6
-
2026年云南大學(xué)青拔課題組招收地理學(xué)，大氣科學(xué)、資源與環(huán)境等專業(yè)研究生
+1/5
-
招調(diào)劑生
+1/5
-
杭州師范大學(xué)-浙江省濕地智慧監(jiān)測(cè)與生態(tài)修復(fù)重點(diǎn)實(shí)驗(yàn)室團(tuán)隊(duì)碩士調(diào)劑公告
+1/5
-
材料與化工 375分 求調(diào)劑
+1/3
★
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
|
這個(gè)好像很難，我查到了2種方法 第一種：ASSAY OF CHALCONE SYNTHASE ACTIVITY BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY，PhytochemistVryo,l . 34,N o. 5, pp. 1225-1229，1993 第二種： CHS[12] chaleone synthase，查兒酮合成酶 Chemicals, [2-14C]Malonyl-CoA (1.71GBq•mmo1-1) was purchased from N.E.N. (Boston, Mass., USA) and [U-14C]phenyl-alanine (16.65GBq•mmol-1) from Amersham (Braunschweig, Germany). 4-Coumaroyl-CoA was synthesized by Dr. P.A. Bäumker (Institut für Botanik, Münster, Germany; Bäumker et al. 1988)[13]. Enzyme preparations. ö Pbenylalanine ammonia-lyase: Leaves were cut into small pieces, frozen at -20°C and homogenized in a prechilled mortar in cold acetone (- 20°C The homogenate was filtered and the residue washed several times with cold acetone. The resulting dry powder was suspended in 0.1 M sodium borate buffer (pH 8.8) containing 5 mM dithioerythritol (DTE). Chalcone synthase. All steps were carried out at 0-4°C Leaf material was homogenized in 0.1 M potassium phosphate buffer (pH 6.8) containing 40 mM ascorbate, 1 mM EDTA, 10 mM thiourea硫脲, 5 mM DTE and 0.25 g Polyclar AT.(g FW)-1 (Serva, Heidelberg, Germany). The homogenate was centrifuged for 10 min at 10 000 g and the supernatant concentrated by ultrafiltration (Diaflo PM 30; Amicon, Witten, Germany). Enzyme assays, The activity of PAL was measured by the method of Amrhein and Zenk (1971). The activity of CHS was determined according to Beerhues and Wiermann (1988)[14], except that the pH of the incubation buffer was 7.8 and the final DTE concentration 0.5 mM. The analysis of the reaction products was performed as described by Lembach et al. (1989)[15]. (CHS activity was determined according to Beerhues and Wiermann (1985)[16]. The analysis of the reaction product was carried out using thin layer chromatography with silica gel plates and hexane: ethyl-acetate: methanol = 50:50:l (v/v/v) as a solvent system. For re-chromatography of the eluted product cellulose plates and 15% (v/v) ethanol were employed.) Protein determination. Protein concentration was measured according to the method of Bradford (1976), as modified by Read and Northcote (1981), using ovalbumin as a standard. Schöpker, H., M. Kneisel, L. Beerhues, et al. Phenylalanine ammonia-lyase and chalcone synthase in glands of Primula kewensis (W. Wats): immunofluorescence and immunogold localization. Planta, 1995. 196(4): 712-719. 13. PA, B., A. S and W. R. Metabolism of ferulic acid sucrose esters in anthers of Tulipa cv. Apeldoorn. II. Highly specific degradation of the esters by different esterase activities. Z. Naturforsch, 1988. 43c: 647-655. |
3樓2014-12-26 14:49:21
2樓2014-09-15 14:32:02
4樓2017-10-18 21:48:59
5樓2018-08-05 13:29:58
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 學(xué)碩274求調(diào)劑 +13 | Li李魚 2026-03-26 | 13/650 |
|
|---|---|---|---|---|
|
|
[考博] 材料專業(yè)申博 +4 | 杜雨婷dyt 2026-03-29 | 4/200 |
|
|
|
[考研] 理學(xué)07化學(xué) 303求調(diào)劑 +4 | 睿08 2026-03-27 | 4/200 |
|
|
|
[考研] 一志愿華中師范化學(xué)332分求調(diào)劑 +3 | Lyy930824@ 2026-03-29 | 3/150 |
|
|
[考研] 085601材料工程找調(diào)劑 +17 | oatmealR 2026-03-29 | 18/900 |
|
|
|
[考研] 293求調(diào)劑 +3 | 末未mm 2026-03-30 | 5/250 |
|
|
|
[考研] 考研調(diào)劑 +5 | Sanmu-124 2026-03-26 | 5/250 |
|
|
|
[考研]
 一志愿武漢理工，總分321，英一數(shù)二，求老師收留。
+11
|
nnnnnnn5 2026-03-25 | 11/550 |
|
|
|
[考研] 一志愿北京理工大學(xué)本科211材料工程294求調(diào)劑 +8 | mikasa的圍巾 2026-03-28 | 8/400 |
|
|
|
[考研] 356求調(diào)劑 +4 | gysy?s?a 2026-03-28 | 4/200 |
|
|
|
[考研] 352分-085602-一志愿985 +5 | 海納百川Ly 2026-03-29 | 5/250 |
|
|
|
[考研] 081200-11408-276學(xué)碩求調(diào)劑 +6 | 崔wj 2026-03-26 | 6/300 |
|
|
|
[考研] 315分求調(diào)劑 +7 | 26考研上岸版26 2026-03-26 | 7/350 |
|
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
|
[考研] 0703化學(xué)338求調(diào)劑！ +6 | Zuhui0306 2026-03-26 | 7/350 |
|
|
|
[考研] 321求調(diào)劑 +6 | Ymlll 2026-03-24 | 6/300 |
|
|
|
[考研] 一志愿天津大學(xué)339材料與化工求調(diào)劑 +3 | 江往賣魚 2026-03-26 | 3/150 |
|
|
|
[考研] 309求調(diào)劑 +4 | gajsj 2026-03-25 | 5/250 |
|
|
|
[考研] 086003食品工程求調(diào)劑 +6 | 淼淼111 2026-03-24 | 6/300 |
|
|
|
[考研]
 085404電子信息284分求調(diào)劑
+4
|
13659058978 2026-03-24 | 4/200 |
|