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lp_0820木蟲(chóng) (著名寫(xiě)手)
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[求助]
有沒(méi)有好的方法測(cè)定液泡和細(xì)胞質(zhì)中K+的濃度 已有1人參與
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專家顧問(wèn) (職業(yè)作家)
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專家經(jīng)驗(yàn): +426 |
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傳統(tǒng)方法為分離出植物細(xì)胞的液泡和胞質(zhì)成分,分別測(cè)定鉀離子濃度即可,參考 Sodium, Potassium, Chloride, and Betaine Concentrations in Isolated Vacuoles from Salt-Grown Atriplex gmelini Leaves Tōru Matoh, Junko Watanabe and Eiichi Takahashi Plant Physiology May 1987 vol. 84 no. 1 173-177 Electrical Potentials and Na, K, and Cl Concentrations in the Vacuole and Cytoplasm of Nitella translucens R. M. SPANSWICK and E. J. WILLIAMS J. Exp. Bot. (1964) 15 (2): 193-200. 近年來(lái)對(duì)于液泡中K+,可以用 PBFI-AM染,參考 Halperin S.J., Lynch J.P. (2003). Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: In vivo measurements using the fluorescent dyes SBFI and PBFI. J. Exp. Bot. 54: 2035–2043. |

木蟲(chóng) (著名寫(xiě)手)
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