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bpx1990新蟲 (初入文壇)
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[交流]
外行新人一個關于動物組織觀察的問題,求助。。 已有2人參與
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鄙人學機械的,因為老板交叉學科的概念被扔向了偏向醫(yī)學的方向。。 最近在做一個關于超聲手術刀效果的項目,有兩個問題求大神們解答: 1、如果用動物組織來進行超聲手術刀切割效果實驗的話,選用什么動物的什么組織比較真實?外行人,怕用不合適的組織鬧笑話。。 2、想觀察一下切割后的刀口附近組織變化情況,請問用普通顯微鏡方便還是電鏡方便?看資料很多都是做石蠟切片,但機械專業(yè)的實驗室有關的設備不好找啊。。拍電鏡倒是相對方便一些,但效果會不會有影響呢? 初來乍到,不知所言,懇請各位大神多多幫忙~拜謝了~ |
新蟲 (初入文壇)
新蟲 (初入文壇)
專家顧問 (著名寫手)
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看了你的帖子,我個人覺得還是做病理切片比較好,或者你們要是有掃面電鏡應該也可以,但由于你設計的是醫(yī)學的課題,所以對于醫(yī)學生而言病理切片是最合適的,而且也能夠對比由于這種超聲刀切割而造成的組織變性情況,打個比方說組織是否結構受損,以及這種損傷是否進一步導致細胞結構改變啊,包括細胞核的變化等等,但是有普通電鏡應該是看不到細胞內(nèi)部,包括掃描電鏡;但是用掃面電鏡的話可以從組織結構和細胞骨架方面做做研究也是可以的。 以上拙見,僅是個人意見,因為確實沒用電鏡做過,所以是否還有其他的研究方式就不太了解了,僅做參考。 |

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what is your purpose for the ultrasonic knife? generally speaking, most of such device aiming at minimizing tissue injury and/or bleeding. you can start with skin, for sure. If you have special issue of concern, for example, you want to see if ultrasound is good for cartilage cutting, or you want to aim at specific tissue, such as liver or nerve, you should design your experiment accordingly. EM is not your first choice at the very beginning. you will need to do light microscopy. I give you an example for you to get a better understanding: if you want to kill a mosquito, can you do it with a bomb? you know, maybe you can, maybe not. But you should try smaller weapon, handy weapon first, right? maybe your hands are better. This do nothing with the 'effect' as you mentioned, this is a question of what are you looking for. wish you all the best. If you really want to do something in this area, you need to read some papers first. try to find some papers in regenerative medicine or histology journals. good luck. |

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