 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 9 | 1/1 | 返回列表 |
| 查看: 1394 | 回復(fù): 8 | |||
[交流]
誰有用熒光探針測過氧化氫的文獻(xiàn)（最好是生物體內(nèi)）
|
| 誰有用熒光探針測過氧化氫的文獻(xiàn)（最好是生物體內(nèi)） |
回復(fù)此樓» 猜你喜歡
一志愿暨大生物學(xué)290求求調(diào)劑
已經(jīng)有8人回復(fù)
-
廈大309分生物與醫(yī)藥調(diào)劑
已經(jīng)有0人回復(fù)
-
生物物理、生物化學(xué)與分子生物學(xué)論文潤色/翻譯怎么收費(fèi)?
已經(jīng)有50人回復(fù)
-
334生物求調(diào)劑
已經(jīng)有0人回復(fù)
-
一志愿中山大學(xué)生物學(xué)，295分，求A區(qū)調(diào)劑
已經(jīng)有2人回復(fù)
-
求助化合物CD數(shù)據(jù)的處理
已經(jīng)有0人回復(fù)
-
廣州醫(yī)科大學(xué)生物醫(yī)學(xué)工程學(xué)院 生物醫(yī)學(xué)工程工學(xué)-083100 招生
已經(jīng)有5人回復(fù)
-
一志愿湖南大學(xué) 生物學(xué)332分 有國獎(jiǎng)+省級
已經(jīng)有1人回復(fù)
-
美國南加大(USC)藥學(xué)院Prof. Yong (Tiger) Zhang課題組誠聘博士后
已經(jīng)有0人回復(fù)
-
湖北工業(yè)大學(xué)食品科學(xué)與工程系課題組招收2026級食品/生物醫(yī)藥碩士生（含調(diào)劑）
已經(jīng)有0人回復(fù)
brilliant
捐助貴賓 (職業(yè)作家)
- 應(yīng)助: 118 (高中生)
- 金幣: 12318.9
- 散金: 262
- 紅花: 47
- 帖子: 4912
- 在線: 1107.8小時(shí)
- 蟲號: 71523
- 注冊: 2005-05-31
- 性別: GG
- 專業(yè): 配位化學(xué)
★ ★ ★
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-28 08:53:33
康潔: 金幣+1 2014-07-23 10:23:15
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-28 08:53:33
康潔: 金幣+1 2014-07-23 10:23:15
2樓2014-04-26 05:55:38
mzhyan
至尊木蟲 (文壇精英)
- 應(yīng)助: 712 (博后)
- 金幣: 37461.7
- 紅花: 24
- 帖子: 45756
- 在線: 287.3小時(shí)
- 蟲號: 1191620
- 注冊: 2011-01-18
- 專業(yè): 環(huán)境污染化學(xué)
3樓2014-04-26 06:56:17
wbshuai
木蟲 (正式寫手)
- 應(yīng)助: 4 (幼兒園)
- 金幣: 8105.7
- 散金: 47
- 紅花: 2
- 帖子: 494
- 在線: 144.9小時(shí)
- 蟲號: 1578513
- 注冊: 2012-01-15
- 性別: GG
- 專業(yè): 高分子合成化學(xué)
4樓2014-04-26 08:16:48
5樓2014-04-27 22:42:29
brilliant
捐助貴賓 (職業(yè)作家)
- 應(yīng)助: 118 (高中生)
- 金幣: 12318.9
- 散金: 262
- 紅花: 47
- 帖子: 4912
- 在線: 1107.8小時(shí)
- 蟲號: 71523
- 注冊: 2005-05-31
- 性別: GG
- 專業(yè): 配位化學(xué)
★ ★ ★ ★ ★ ★ ★
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-29 09:19:52
康潔(likedong1021代發(fā)): 金幣+5, 感謝回帖交流！ 2014-07-28 04:51:19
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-29 09:19:52
康潔(likedong1021代發(fā)): 金幣+5, 感謝回帖交流！ 2014-07-28 04:51:19
|
Nature Protocols | Protocol PrintEmailShare/bookmark Cite U LikeFacebookTwitterDeliciousDiggGoogle+LinkedInRedditStumbleUponPrevious articleNature Protocols | Protocol Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry Next articleNature Protocols | Protocol In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence probe Xiaoming Zhou,1, Debin Zhu,1, Yuhui Liao,1, Weipeng Liu,1, Hongxing Liu,1, Zhaokui Ma1, & Da Xing1, AffiliationsContributionsCorresponding author Journal name: Nature Protocols Volume: 9, Pages: 1146–1159 Year published: (2014)DOI: doi:10.1038/nprot.2014.060 Published online17 April 2014 Article toolsFull text Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence prob... Download as PDF (921 KB) View interactive PDF in ReadCube CitationReprintsRights & permissionsArticle metrics Abstract Abstract• References• Author information• Supplementary information Assays using probes labeled with electrochemiluminescent moieties are extremely powerful analytical tools that are used in fields such as medical diagnostics, environmental analysis and food safety monitoring, in which sensitive, reliable and reproducible detection of biomolecules is a requirement. The most efficient electrochemiluminescence (ECL) reaction to date is based on tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) with tripropylamine (TPrA) as the co-reactant. Here we present a detailed protocol for preparing Ru(bpy)32+ probes and their bioanalytical applications. This protocol includes (i) the synthesis of a biologically active Ru(bpy)32+-N-hydroxysuccinimide (NHS) ester, (ii) its covalent labeling with both antibodies and DNA probes and (iii) the detection and quantification of ECL in a microfluidic system with a paramagnetic microbead solid support. In our magnetic bead–based ECL system, two probes are required: a capture probe (labeled with biotin to be captured by a streptavidin-coated magnetic bead) and a detector probe (labeled with Ru(bpy)32+). The complex consisting of the analyte, the capture probe, the detector probe and the magnetic bead is brought into contact with the electrode by using a magnetic field. The Ru(bpy)32+ reacts with TPrA in solution to generate the ECL signal. The full protocol, including the synthesis and labeling of the bioactive Ru(bpy)32+, requires 5–6 d to complete. ECL immunoassays or nucleic acid tests only require 1.5–2 h, including the sample preparation time. |
6樓2014-04-28 09:28:22
zqx1990
木蟲 (正式寫手)
- 應(yīng)助: 49 (小學(xué)生)
- 金幣: 4866.7
- 散金: 350
- 紅花: 7
- 帖子: 723
- 在線: 600.9小時(shí)
- 蟲號: 1264137
- 注冊: 2011-04-13
- 性別: GG
- 專業(yè): 生物醫(yī)用高分子材料
★ ★
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-29 09:20:57
小木蟲: 金幣+0.5, 給個(gè)紅包，謝謝回帖
黃酮: 金幣+1, 謝謝回帖交流 2014-04-29 09:20:57
7樓2014-04-28 22:00:48
zqx1990
木蟲 (正式寫手)
- 應(yīng)助: 49 (小學(xué)生)
- 金幣: 4866.7
- 散金: 350
- 紅花: 7
- 帖子: 723
- 在線: 600.9小時(shí)
- 蟲號: 1264137
- 注冊: 2011-04-13
- 性別: GG
- 專業(yè): 生物醫(yī)用高分子材料
★ ★ ★ ★ ★
黃酮: 金幣+1, 應(yīng)助指數(shù)+1, 謝謝回帖交流 2014-04-29 09:20:37
康潔(likedong1021代發(fā)): 金幣+4 2014-07-28 04:51:35
黃酮: 金幣+1, 應(yīng)助指數(shù)+1, 謝謝回帖交流 2014-04-29 09:20:37
康潔(likedong1021代發(fā)): 金幣+4 2014-07-28 04:51:35
|
In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles Dongwon Lee1,5, Sirajud Khaja1,5, Juan C. Velasquez-Castano2, Madhuri Dasari1, Carrie Sun3, John Petros3,4, W. Robert Taylor1,2,4 & Niren Murthy1 Abstract The overproduction of hydrogen peroxide is implicated in the development of numerous diseases1, 2, 3, 4 and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalate nanoparticles image hydrogen peroxide by undergoing a three-component chemiluminescent reaction between hydrogen peroxide, peroxalate esters and fluorescent dyes. The peroxalate nanoparticles have several attractive properties for in vivo imaging, such as tunable wavelength emission (460–630 nm), nanomolar sensitivity for hydrogen peroxide and excellent specificity for hydrogen peroxide over other reactive oxygen species. The peroxalate nanoparticles were capable of imaging hydrogen peroxide in the peritoneal cavity of mice during a lipopolysaccharide-induced inflammatory response. We anticipate numerous applications of peroxalate nanoparticles for in vivo imaging of hydrogen peroxide, given their high specificity and sensitivity and deep-tissue-imaging capability. |
8樓2014-04-28 22:01:41
zqx1990
木蟲 (正式寫手)
- 應(yīng)助: 49 (小學(xué)生)
- 金幣: 4866.7
- 散金: 350
- 紅花: 7
- 帖子: 723
- 在線: 600.9小時(shí)
- 蟲號: 1264137
- 注冊: 2011-04-13
- 性別: GG
- 專業(yè): 生物醫(yī)用高分子材料
9樓2014-04-28 22:03:05
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 學(xué)員9yipfZ 的主題更新
| 9 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 本科鄭州大學(xué)物理學(xué)院，一志愿華科070200學(xué)碩，346求調(diào)劑 +3 | 我不是一根蔥 2026-03-18 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 一志愿中海洋材料工程專碩330分求調(diào)劑 +5 | 小材化本科 2026-03-18 | 5/250 |
|
|
|
[考研] 311求調(diào)劑 +6 | 26研0 2026-03-15 | 6/300 |
|
|
|
[考研] 298-一志愿中國農(nóng)業(yè)大學(xué)-求調(diào)劑 +7 | 手機(jī)用戶 2026-03-17 | 7/350 |
|
|
|
[考研] 收復(fù)試調(diào)劑生 +4 | 雨后秋荷 2026-03-18 | 4/200 |
|
|
|
[考研] 268求調(diào)劑 +7 | 好運(yùn)連綿不絕 2026-03-12 | 8/400 |
|
|
|
[考研] 268求調(diào)劑 +8 | 一定有學(xué)上- 2026-03-14 | 9/450 |
|
|
|
[考研] 332求調(diào)劑 +6 | Zz版 2026-03-13 | 6/300 |
|
|
|
[考博] 26申博 +4 | 八旬速覽 2026-03-16 | 4/200 |
|
|
|
[考研] [導(dǎo)師推薦]西南科技大學(xué)國防/材料導(dǎo)師推薦 +3 | 尖角小荷 2026-03-16 | 6/300 |
|
|
|
[考研] 085600調(diào)劑 +5 | 漾漾123sun 2026-03-12 | 6/300 |
|
|
|
[考研] 求老師收留調(diào)劑 +4 | jiang姜66 2026-03-14 | 5/250 |
|
|
|
[考研] 085601材料工程315分求調(diào)劑 +3 | yang_0104 2026-03-15 | 3/150 |
|
|
|
[考研] 297求調(diào)劑 +4 | 學(xué)海漂泊 2026-03-13 | 4/200 |
|
|
|
[考研] ［0860］321分求調(diào)劑，ab區(qū)皆可 +4 | 寶貴熱 2026-03-13 | 4/200 |
|
|
|
[考研] 0856材料與化工301求調(diào)劑 +5 | 奕束光 2026-03-13 | 5/250 |
|
|
|
[考研] 求調(diào)劑 +5 | 一定有學(xué)上- 2026-03-12 | 5/250 |
|
|
|
[考研] 0703化學(xué)求調(diào)劑 +7 | 綠豆芹菜湯 2026-03-12 | 7/350 |
|
|
|
[考研] 295求調(diào)劑 +3 | 小匕仔汁 2026-03-12 | 3/150 |
|
|
|
[考研] 一志愿山大07化學(xué) 332分 四六級已過 本科山東雙非 求調(diào)劑！ +3 | 不想理你 2026-03-12 | 3/150 |
|