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下面是我自己翻譯的,老師說太中文化了,求幫助修改下,或從新翻譯, 謝謝啦。! 摘要:通過已知擬南芥的生長素結(jié)合蛋白(ABP1)基因序列和禾本科物種高粱、水稻、玉米、二葉短柄草的全基因組序列進(jìn)行比較分析,在4個禾本科物種中鑒定其他的生長素結(jié)合蛋白基因家族并對ABP1基因家族進(jìn)行序列比對分析、共線性分析、基因家族的系統(tǒng)進(jìn)化發(fā)育分析研究該基因家族保守結(jié)構(gòu)域及作用機(jī)制和該基因家族的遺傳進(jìn)化機(jī)制。結(jié)果顯示,擬南芥、高粱、水稻、玉米、二葉短柄草ABP1蛋白中有12個同源性較高的序列基因,其多數(shù)定位于內(nèi)質(zhì)網(wǎng)上,少數(shù)位于等離子體膜上;除At4g19647295編碼的蛋白跨膜外,其余皆不跨膜。12個蛋白結(jié)構(gòu)保守性較強(qiáng),都有Auxin_BP和Cupin_2倆個保守域位置,并分成2個亞家族。 關(guān)鍵詞:ABP1,禾本科物種,擬南芥,結(jié)構(gòu)預(yù)測,進(jìn)化分析 Abstract: compare gene sequence of the Arabidopsis thaliana’s auxin binding protein (ABP1) with the whole genome sequence of the four grasses, sorghum bicolor, oryza sativa, zea mays and Brachypodium discolor .Then establish auxin binding protein gene family and use the ABP1 gene family for sequence alignment analysis, linear analysis, evolution development domain analysis to study conservative structure, mechanism of action and the genetic evolutionary mechanism of this gene family. The results showed that there are 12 members in this ABP1 gene family , and most of its location in the endoplasmic reticulum, a few on the plasma membrane; Besides the proteins encoded by At4g19647295 across the membrane, the rest are not .The conservative of these 12 protein structure is stronger, and they all possess Auxin_BP and Cupin_2 these two conservative domain; And divided into two subfamily. Keywords: ABP1, grasses, Arabidopsis thaliana, prediction of structure, evolutionary analysis |
版主 (文壇精英)
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Abstract:The known gene sequences of Arabidopsis auxin binding protein (ABP1) were compared with the whole genome sequences of the four Poaceae species of sorghum vulgar , rice/oryza sativa, maize, and two leave Brachypodium distachyon .Other auxin binding protein of the four Poaceae species were identified and sequence alignment , synteny ,and gene family phylogenetic analysis of ABP1 gene family were performed to investigate the gene conserved domain and its action mechanism ,and ,genetic evolution mechanism . The results showed that Arabidopsis, sorghum, rice, maize, two leave Brachypodium ABP1 protein have 12 high homology sequence gene, the majority of which located in the endoplasmic reticulum, a few in the plasma membrane.Except At4g19647295 encoded protein, the remaining are not transmembrane protein. All 12 proteins are highly conserved structure and all have Auxin_BP and Cupin_2 two conserved domain and divided into two subfamilies. Keywords: ABP1, Poaceae species , Arabidopsis thaliana, structure prediction, evolution analysis |

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