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lijun585429銅蟲 (初入文壇)
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[求助]
文章投稿后一個(gè)月，收到編輯的大修意見
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第一次投英文文章，投稿后一個(gè)月收到編輯的回復(fù)了，建議大修。一共找了兩個(gè)審稿人，第一個(gè)意見比較尖銳不建議接收；第二個(gè)比較nice一些，提了6個(gè)問題，但是都是比較positive的，一些建議什么的。 另附上reviewer1的意見大家?guī)兔Ψ治鲆幌略撊绾位貜?fù)，非常感謝了。 Reviewer #1: General comments This study investigated “文章標(biāo)題...." 。As the results, hPDLCs conditioned by” 一種物質(zhì)“ demonstrated a significant increase in proliferation and also showed an increased formation of mineralized matrix nodules and increased ALP activity with the attendant expression of Runx2. Based on these findings, the authors suggested that ”一種物質(zhì)“ induces proliferation and osteogenic differentiation of hPDLCs, indicating that”一種物質(zhì)“ may be applicable as a novel autologous graft in periodontal tissue engineering regeneration. The aims of this study are clear and the results obtained are interesting. However, the current findings appeared to quite preliminary data. Without the data showing the possible mechanisms by which”一種物質(zhì)“ stimulated proliferation and osteoblast differentiation of hPDLCs, it can't be further considerable for acceptance. Specific comments First of all, there was no a study to verify the mechanisms involved in the ”一種物質(zhì)“-mediated increases in proliferation and differentiation of the cells. It is strongly recommended to show why the blood samples did so in the hPDLCs. Especially, to end this, the authors have to try to check cell cycle progression using flow cytometer and then to analyze what are the regulatory factors such as CDKs and/or cyclins are involved therein by Western blot analysis. In addition, the authors showed the expression pattern of Runx2 as an osteogenic marker. Of course, this factor might be one of the most important osteogenic factors. However, it will be needed to show that which of the signaling molecules are closely associated with the induction of Runx2 as a second messenger by treating the cells with the blood samples. With these additional experiments, the authors are also capable of explaining the reason why the ”一種物質(zhì)“ among the samples showed the most prominent activities. Minor comments 1. The figures 2 and 3 are not necessary in this manuscript, rather it is recommended to show as a supplemental figure. 2. Please check carefully misspelling (ex, HPDLCs -> hPDLCs) and grammatical errors. 3. The length of several sentences shown in the highlights appeared to be over a general limitation. Please check this with consideration of the Journal Policy. 總結(jié)起來，這位reviewer的意見就是集中在該文章未做機(jī)制方面的研究。事實(shí)上我也確實(shí)沒有再對(duì)此深入下去，這方面的文章很少做到機(jī)制層面上的，因?yàn)樵撐镔|(zhì)成分比較復(fù)雜，碩士期間也沒能力在這方面深入下去，不過我以后倒是有這方面的想法。我也知道自己文章比較淺顯所以才投的這個(gè)雜志(IF=1.5)，如果做到機(jī)制方面的話，肯定會(huì)投3分以上的文章了。 現(xiàn)在不知道如何回復(fù)這個(gè)reviewer，編輯給我修回的時(shí)間是4周。 |
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