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[求助]
求助,枯草營(yíng)養(yǎng)培養(yǎng)基 已有2人參與
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最近在看關(guān)于枯草的書(shū),有一段話摘抄自營(yíng)養(yǎng)培養(yǎng)基一節(jié)。不是很理解。麻煩哪位大俠點(diǎn)撥一下!謝謝! Because of their rather granular consistency, colonies of B. subtilis grown on simple nutrient agar plates can be difficult to use consistently for inoculating other cultures. This is particularly noticeable when inoculating liquid cultures, since clumps of cells often remain intact, increasing in size as the culture grows and producing very heterogeneous cultures. These problems may be overcome to some extent by the use of a straight wire rather than a loop for inoculation. The sides of the tube or flask just above the surface of the médium should be wetted with a little médium and the inoculating cell mass macérated to a smooth paste with the wire. Difficul- ties can also be experienced when testing the phenotype of colonies by spot inoculating from nutrient agar plates, since the tendency to clump leads to biomass growth at the site of inoculation. Clones can be falsely scored as positive even w h e n antibiotic selection is used. |
捐助貴賓 (著名寫(xiě)手)
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樓主給出的這段英文的意思于下: 由于枯草桿菌的成粒粘滯性導(dǎo)致從基本培養(yǎng)基上的菌落用于均勻接種困難, 這在將其接種到液體培養(yǎng)基中時(shí)應(yīng)尤其注意, 因?yàn)槌?dǎo)致接種后不均勻生長(zhǎng), 可用接種針代替接種環(huán)接種, 并且在接入瓶培養(yǎng)液上方的瓶壁上浸濕接入的細(xì)胞團(tuán)可在一定程度上克服接種后成團(tuán)的現(xiàn)象。當(dāng)用營(yíng)養(yǎng)瓊脂上的菌作菌種點(diǎn)接后檢測(cè)菌落特征時(shí), 也存在生長(zhǎng)不均的現(xiàn)象。即使在抗生素篩選實(shí)驗(yàn)中, 由于枯草桿菌細(xì)胞粘滯成團(tuán), 也導(dǎo)致出現(xiàn)假陽(yáng)性現(xiàn)象。 祝您成功! |

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