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[求助]
麻煩看看這篇論文是不是已經被SCI收錄了!。 已有1人參與
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哪位所在的學校能上web of science, 能幫我看看,下面這篇論文是不是已經被SCI收錄了? 這篇論文是2月份發(fā)表的,發(fā)表的期刊 Anal Biochem.是SCI全文收錄的。 但是從發(fā)表到被SCI收錄是需要有個時間的,也許。 現(xiàn)在學校讓開一個SCI收錄證明,挺著急。 可是在我們學校又不能上web of science. 看不到此刻是不是已經被收錄了。 哪位能好心幫忙看看是不是已經被SCI收錄了? 謝謝哦。 Gao S, Li Y, Zhang J, Chen H, Ren D, Zhang L, An Y. A modified version of the digestion-ligation cloning method for more efficient molecular cloning. Anal Biochem. 2014 453: 55-57. [Epub ahead of print] |
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A modified version of the digestion-ligation cloning method for more efficient molecular cloning 作者:Gao, S (Gao, Song)[ 1 ] ; Li, YL (Li, Yanling)[ 1 ] ; Zhang, JN (Zhang, Jiannan)[ 1 ] ; Chen, HM (Chen, Hongman)[ 1 ] ; Ren, DM (Ren, Daming)[ 1 ] ; Zhang, LJ (Zhang, Lijun)[ 1 ] ; An, YF (An, Yingfeng)[ 1 ] ANALYTICAL BIOCHEMISTRY 卷: 453 頁: 55-57 DOI: 10.1016/j.ab.2014.02.031 出版年: MAY 15 2014 查看期刊信息 摘要 Here we describe a modified version of the digestion-ligation approach for efficient molecular cloning. In comparison with the original method, the modified method has the additional steps of gel purification and a second ligation after the first ligation of the linearized vector and DNA insert. During this process, the efficiency and reproducibility could be significantly improved for both stick-end cloning and bluntend cloning. As an improvement of the very important molecular cloning technique, this method may find a wide range of applications in bioscience and biotechnology. (C) 2014 Elsevier Inc. All rights reserved. 關鍵詞 作者關鍵詞:Blunt-end DNA ligation; T4 DNA ligase; Molecular cloning; Digestion-ligation approach KeyWords Plus:INDEPENDENT CLONING; PCR PRODUCTS; ENZYME-FREE; DNA; LIGASE 作者信息 通訊作者地址: An, YF (通訊作者) Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110161, Peoples R China. 地址: [ 1 ] Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110161, Peoples R China 電子郵件地址:anyingfeng666@163.com 基金資助致謝 基金資助機構 授權號 National Natural Science Foundations of China 31100045 31270114 Program for Liaoning Excellent Talents in University LJQ2011067 查看基金資助信息 出版商 ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA 類別 / 分類 研究方向:Biochemistry & Molecular Biology; Chemistry Web of Science 類別:Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical 文獻信息 文獻類型:Article 語種:English 入藏號: WOS:000335488400010 ISSN: 0003-2697 電子 ISSN: 1096-0309 其他信息 IDS 號: AG5WC Web of Science 核心合集中的 "引用的參考文獻": 15 Web of Science 核心合集中的 "被引頻次": 0 |
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