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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
yaoguiyang木蟲(chóng) (小有名氣)
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[求助]
求翻譯一段摘要
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The growth of stem cells can be modulated by physical factors such as extracellular matrix nanotopography. We hypothesize that nanotopography modulates cell behavior by changing the integrin clustering and focal adhesion (FA) assembly, leading to changes in cytoskeletal organization and cell mechanical properties. Human mesenchymal stem cells (hMSCs) cultured on 350 nm gratings of tissueculture polystyrene (TCPS) and polydimethylsiloxane (PDMS) showed decreased expression of integrin subunits a2, a6, aV, b2, b3 and b4 compared to the unpatterned controls. On gratings, the elongated hMSCs exhibited an aligned actin cytoskeleton, while on unpatterned controls, spreading cells showed a random but denser actin cytoskeleton network. Expression of cytoskeleton and FA components was also altered by the nanotopography as reflected in the mechanical properties measured by atomic force microscopy (AFM) indentation. On the rigid TCPS, hMSCs on gratings exhibited lower instantaneous and equilibrium Young’s moduli and apparent viscosity. On the softer PDMS, the effects of nanotopography were not significant. However, hMSCs cultured on PDMS showed lower cell mechanical properties than those on TCPS, regardless of topography. These suggest that both nanotopography and substrate stiffness could be important in determining mechanical properties, while nanotopography may be more dominant in determining the organization of the cytoskeleton and FAs. |
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reko34
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| 可通過(guò)例如細(xì)胞外基質(zhì)納米形貌的物理因素調(diào)節(jié)干細(xì)胞的生長(zhǎng)。我們假設(shè)納米形貌通過(guò)改變整合素聚集和粘著斑（FA）集合調(diào)節(jié)細(xì)胞行為，導(dǎo)致細(xì)胞骨架組織和細(xì)胞機(jī)械性能變化。與非模式化的控制相比，在組織培養(yǎng)聚苯乙烯(TCPS)和聚二甲基硅氧烷（PDMS）的350nm格柵上培養(yǎng)得到的人間充質(zhì)干細(xì)胞（hMSCs）上，整合素的子成分a2、a6、aV、b2、b3和b4的出現(xiàn)減少。格柵上，延長(zhǎng)了的hMSCs展現(xiàn)出對(duì)齊的肌動(dòng)蛋白細(xì)胞骨架，而在非模式化控制下，擴(kuò)散細(xì)胞展現(xiàn)出隨機(jī)但較密的肌動(dòng)蛋白細(xì)胞骨架網(wǎng)絡(luò)。細(xì)胞骨架和FA成分的表現(xiàn)度也被納米形貌改變，如原子力顯微鏡(AFM)測(cè)量的機(jī)械性能所反映的。在剛性TCPS上，格柵上的hMSCs顯示出較低的瞬態(tài)和穩(wěn)態(tài)楊氏模量及明顯的粘性。在較軟的PDMS上，納米形貌的作用不顯著。但PDMS上培養(yǎng)的hMSCs與TCPS上的相比，細(xì)胞機(jī)械性能較低，不論地貌如何。這些結(jié)果顯示，納米形貌和基板硬度都對(duì)機(jī)械性能起到重要作用，其中納米形貌在決定細(xì)胞骨架和FA組織上起主導(dǎo)作用。 |
2樓2014-06-19 11:12:14
yaoguiyang
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3樓2014-06-19 13:36:07
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