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wenkoll銅蟲 (初入文壇)
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[求助]
跪求Griess reagent (modified)的使用 已有1人參與
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| 大家好,小弟在做一氧化氮自由基清除實驗,老板買了Sigma 的Griess reagent (modified),以前沒用過,查了很多文獻也沒找到用這個做實驗的,有哪位哥哥姐姐用過Griess reagent (modified),幫幫忙,分享一下你的操作方法,特別是Griess reagent的濃度以及用什么溶液配置。非常感謝 |

木蟲 (小有名氣)
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Griess試劑盒的使用說明書 http://pan.baidu.com/s/1i35O58p |
金蟲 (正式寫手)
好樣的
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Nitrite (NO2–) detection by Griess Reagent. 50 μL of the sample was taken and transferred into a cuvette and combined with 50 μL of Griess Reagent and 0.9 mL of Milli-Q water. The resulting sample was mixed thoroughly. The mixture was incubated at room temperature in the dark for 0.5 h prior to UV-Vis testing. Solutions of NaNO2 with known concentrations (in 10 mM H2SO4) were used as calibration standards, with the absorbance at 525 nm used to plot the calibration curves. Upper LOD of UV-Vis used in this study refers to the absorbance at 525 nm obtained from 200 μM NaNO2. A dilution factor was applied to measure nitrite concentration in plasma-activated water (PAW) |

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