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[求助]
求助文獻(xiàn)中突變體的表示是什么意思 已有1人參與
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| in tmk1-/+tmk234 and tmk1-/-tmk234 mutants,這前面TMK后后面帶的符號表示什么意思。縜nd abp1-5;tmk1-/-tmk234,表示一個突變體植株,這是三倍突變體還是雙突變體或是其他的什么?菜鳥求大神指教! |
分子生化實驗經(jīng)驗積累 |
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突變體書寫格式: http://www.biolreprod.org/site/IforA/msprep/nomenbullets.xhtml Biology of Reproduction’s Nomen Bullets: Nomenclature Guidelines and Requirements PLEASE NOTE: BOR no longer provides nomenclature editing for accepted papers; it is the authors' responsibility to ensure that their nomenclature is correct prior to publication. BOR, like many major journals, requires the use of official approved names and symbols for proteins, genes, alleles, and strains in all sections of the paper, including the title and figures. Most gene expression and gene ontology databases use these official names and symbols. Do not assume that names and symbols in the literature are correct. Use species-appropriate databases (see below) to ensure that names and symbols are correct for each gene, transcript, and protein. If the official gene/protein name or symbol is different from an earlier name, at the first use of the official name, add in parentheses, "(previously known as xxx)"; e.g., "… we used antibodies against the POU5F1 protein (previously known as OCT4)…." Use only the correct symbol from then on. Nomenclature Guidelines for Specific Species A. Mouse and Rat Websites for nomenclature rules: Mouse and rat: http://www.informatics.jax.org Rat only: http://rgd.mcw.edu General nomenclature rules (mouse and rat): Full gene names are in roman font (not italic); e.g., insulin-like growth factor 1. Greek symbols are not used. Gene, mRNA, and cDNA symbols: Italic font, with only the first letter upper case; e.g., Igf1. Greek symbols are not used. Hyphens are rarely used. Protein symbols: Use the same symbol as the gene. Roman font (not italic), with all letters upper case; e.g., IGF1. Mutant alleles: Define when first mentioned; e.g., "Igf1 tm1Arge/Igf1 tm1Arge is one of several knockout alleles of Igf1." Italic font is used for all letters and numbers, with the allelic designation (e.g., tm1Arge) in superscript. After first mention, the homozygous KO can be indicated as Igf1-/-; the heterozygote can be indicated as Igf1+/-, etc. For more details, see: MGI Nomenclature MGI Quick Guide for Genes B. Humans, nonhuman primates, chickens, domestic species, and everything that is not a mouse, rat, fish, worm, frog, or fly Website for nomenclature rules: http://www.genenames.org General nomenclature rules: Full gene names are in roman font (not italic); e.g., insulin-like growth factor 1. Greek symbols are not used. Gene, MRNA, and cDNA symbols: Italic font, with all letters upper case; e.g., IGF1. Greek symbols are never used. Hyphens are used only in very specific cases (please refer to the nomenclature guidelines). Protein symbols: Use the same symbol as the gene. Roman font (not italic), with all letters upper case; e.g., IGF1. C. Fish (applies to all fish) Website for nomenclature rules: http://zfin.org/cgi-bin/webdriver?MIval=aa-ZDB_home.apg. General nomenclature rules: Full gene names are in italic font, with all letters lower case; e.g., cyclops. Greek symbols are not used. Gene symbols: Italic font, with all letters lower case; e.g., cyc. Greek symbols are not used. Protein symbols: Use the same symbol as the gene. Roman font (not italic), with only the first letter upper case; e.g., Cyc. |

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