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[求助]
檢測藥物在細菌亞結(jié)構(gòu)中的分布方法 已有1人參與
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想檢測一個藥物在細菌亞結(jié)構(gòu)中的分布,只要是在細胞壁和細胞質(zhì)間的分布?吹揭粋實驗方法,但是不知道其中的“saccarose”是什么東西,也沒找到哪有賣的,原文如下: The subcellular distribution of porphyrins was evaluated after fractionation of the cells. A pellet of l0(8) cells was resuspended in 1 ml of a solution containing Tris-HC 0.05 M, pH 7.2, 0.5 M saccarose, 0.01 M Mg-acetate and 1 mg lysozyme (for G+ bacteria) and Tris-HCl 0.05 M, pH 6.8, 0.3 M saccarose, 0.01 M EDTA and 1 mg lysozyme (for G- bacteria). After 30min (G+) or 90 min (G-) incubation at 37 °C with gentle magnetic stirring the cell suspensions were centrifuged at 3000×g for 10 min. The pellets containing either the spheroplasts or the protoplasts were resuspended in 3 ml of 2% aqueous SDS, while the sumatants (about 1 ml) containing the cell wall were diluted with 2 ml of 3% aqueous SDS. 希望能幫著解答一下,或者有別的方法可以提供! 謝謝了! |
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