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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
tianlus1632木蟲 (正式寫手)
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[求助]
【農(nóng)業(yè)類】求大神幫忙翻譯一段摘要（中譯英），非常急用！先謝謝！
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| 以雅安黃油菜開花后10 d的根、莖、葉、花、種皮等不同組織器官和開花后10 d、20 d、30 d、40 d、50 d、60 d、70 d（收獲后10 d）的種皮為研究材料，通過實(shí)時(shí)熒光定量（qPCR）方法對(duì)類黃酮途徑相關(guān)結(jié)構(gòu)基因和調(diào)控因子進(jìn)行表達(dá)分析。研究發(fā)現(xiàn)：（1）類黃酮途徑基因表達(dá)總量在花中最高，在根和莖中較少，但黃、褐籽間無明顯差異；在葉和開花后10 d的種皮中，類黃酮途徑各基因表達(dá)總量較高，且褐籽明顯高于黃籽。（2）在雅安黃油菜種皮的整個(gè)發(fā)育時(shí)期，黃籽中類黃酮合成途徑各基因表達(dá)總量呈下降趨勢，前、中期下降較快，后期趨于平穩(wěn)；褐籽種皮發(fā)育的前、中期，類黃酮合成途徑各基因的表達(dá)量逐漸下降，50 d后（即種子成熟階段），基因的表達(dá)總量迅速升高；20 d、60 d和70 d時(shí)，黃、褐籽間類黃酮合成途徑各基因的表達(dá)量差異明顯，且越往后差異越大。（3）種皮發(fā)育不同時(shí)期，黃、褐籽基因表達(dá)量差異主要集中在BraANR（LDOX）和BraANS（BAN）基因；20 d時(shí)褐籽BraANR基因?yàn)辄S籽的38.14倍，60 d和70 d時(shí)褐籽BraANS基因分別為黃籽的49.93倍和81.58倍。研究認(rèn)為，雅安黃油菜黃、褐籽種皮顏色的差異受整個(gè)類黃酮合成途徑各基因的協(xié)同影響，但BraANR（LDOX）和BraANS（BAN）這兩個(gè)基因在其中起到了十分重要的作用。 |
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tianlus1632
木蟲 (正式寫手)
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- 注冊: 2008-10-10
- 專業(yè): 作物種質(zhì)資源與遺傳育種學(xué)
2樓2014-10-23 09:28:20
LifePursuit
木蟲之王 (文學(xué)泰斗)
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
tianlus1632: 金幣+100, 翻譯EPI+1, ★★★★★最佳答案, 謝謝！翻譯的很不錯(cuò)，只是有兩個(gè)句子的意思和中文有點(diǎn)出入，還是謝謝了！ 2014-10-23 11:50:10
phu_grassman: 金幣+2, good job. 2014-10-23 16:58:01
tianlus1632: 金幣+100, 翻譯EPI+1, ★★★★★最佳答案, 謝謝！翻譯的很不錯(cuò)，只是有兩個(gè)句子的意思和中文有點(diǎn)出入，還是謝謝了！ 2014-10-23 11:50:10
phu_grassman: 金幣+2, good job. 2014-10-23 16:58:01
| We conducted expression analysis on the flavonoid synthetic pathway related structural genes and regulation factors by real-time fluorescence quantitative PCR (qPCR) assay based on various tissue organs (e.g., roots, stems, leaves, blossoms and seed coats) of Brassica rapa L. (Ya'an Yellow Rape) 10 d after blossoming, as well as seed coats 10 d, 20 d, 30 d, 40 d, 50 d, 60 d and 70 d after blossoming (10 d after being harvested). Our findings include: (1) The total expression of flavonoid pathway genes is the highest in blossoms while the lowest in roots and stems, and there is no significant expression difference between yellow and brown seeds. Besides, the total expression in seed coats reaches its maximum 10 d after blossoming, and it is significantly higher in brown seeds than in yellow seeds. (2) The expression of flavonoid synthetic pathway related genes in yellow seeds decreases during the whole developmental stages of seed coats, and the decreasing rate is fairly high during the early and middle stages while steady during the late stage. Moreover, the expression in brown seeds decreases steadily during the early and middle stages while increases rapidly from 50 d after blossoming (i.e., seed mature stage). There is a significant expression difference between yellow and brown seeds 20 d, 60 d and 70 d after blossoming, and this difference gets increasing with the increase of time. (3) The expression difference between yellow and brown seeds concentrates on BraANR (LDOX) and BraANS (BAN) genes during the whole developmental stages of seed coats. More concretely, the expression of BraANR (LDOX) gene in brown seeds is 38.14, 49.93 or 81.58 times that in yellow seeds 20 d, 60 d or 70 d after blossoming, respectively. We conclude that, with these observations, there is a synergistic effect among all flavonoid synthetic pathway related genes on the color difference of brown and yellow seeds, while BraANR (LDOX) and BraANS (BAN) genes are the most prominent in this synergistic effect. |
3樓2014-10-23 11:11:36
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