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raohuihua新蟲 (小有名氣)
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[求助]
求助翻譯一段英文
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Proteomic analysis of untreated stationary cells led to the detection of 1712 proteins (65% of the predicted ORFs). Treatment with ADEP4 resulted in decreased abundance of 243 proteins (p≤0.05 and 2-fold decrease) (Fig 1A) (Extended Data Table 1). This however is likely an underestimate. The proteome reports changes in the relative abundance of peptides produced by trypsin cleavage. A protein that was only cleaved once, for example, by ADEP4/ClpP and was not further degraded prior to trypsin treatment would still generate several tryptic peptides and not show an overall decrease in protein abundance. To address this, we analyzed partially-tryptic peptides to uncover additional ADEP/ClpP targets. Partially tryptic peptides exist at certain abundance in untreated cells due to natural degradation, followed by trypsin treatment. However, the levels of these peptides increases or decreases markedly due to degradation induced by addition of ADEP; as is obvious from the abundance of red spots. An increase of partially tryptic peptides indicates ADEP degradation of a protein resulting in partially tryptic peptides. This analysis revealed 174 additional ADEP/ClpP targets (peptides of increased abundance; Fig 1B; Extended Data Table 2). A decrease on the other hand indicates that a particular degradation product, present at the time of ADEP addition, can be further degraded by ADEP/ClpP and are of less relevance to the study. |
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2樓2014-10-23 16:01:34
ssssllllnnnn 
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【答案】應(yīng)助回帖
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raohuihua: 金幣+15, 翻譯EPI+1, ★有幫助 2014-10-24 09:57:42
raohuihua(RXMCDM代發(fā)): 金幣+15 2014-10-24 23:03:34
raohuihua: 金幣+15, 翻譯EPI+1, ★有幫助 2014-10-24 09:57:42
raohuihua(RXMCDM代發(fā)): 金幣+15 2014-10-24 23:03:34
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（教科書式的翻譯：） 對(duì)未處理的靜止細(xì)胞進(jìn)行蛋白質(zhì)組分析檢測(cè)到1712種蛋白質(zhì)（預(yù)測(cè)ORF【開(kāi)放閱讀框】的65％）。用ADEP4（�；s肽）處理后有243種蛋白質(zhì)的豐度下降（P≤0.05，降低2倍【有人說(shuō)中文不這么說(shuō)】）（圖1A）（擴(kuò)展數(shù)據(jù)見(jiàn)表1）。但這很可能是低估了。（因?yàn)椋┑鞍踪|(zhì)組的報(bào)告的是胰蛋白酶裂解產(chǎn)生的肽段相對(duì)豐度的變化。舉例來(lái)說(shuō)，如果一種蛋白僅被ADEP4/ CLPP裂解一次，在經(jīng)胰蛋白酶處理之前沒(méi)有進(jìn)一步降解（發(fā)生），（但經(jīng)）胰蛋白酶處理后仍然將產(chǎn)生幾個(gè)胰蛋白酶裂解肽段，卻不能顯示蛋白質(zhì)整體豐度的下降。為了解決這個(gè)問(wèn)題，我們分析了用胰蛋白酶部分消化所產(chǎn)生的肽段，以便發(fā)現(xiàn)更多（額外）ADEP/CLPP靶位。用胰蛋白酶處理未經(jīng)處理的細(xì)胞，這些細(xì)胞由于自然降解會(huì)有一定豐度的胰蛋白酶部分消化肽段存在。但加入ADEP后引起蛋白質(zhì)的進(jìn)一步降解，使這些肽段顯著增加或者減少，這可從圖中紅點(diǎn)的豐度明顯看出。胰蛋白酶部分降解肽段的增加表明ADEP對(duì)蛋白質(zhì)的降解，產(chǎn)生部分裂解肽段。此分析發(fā)現(xiàn)了另外174種ADEP/ CLPP目標(biāo)蛋白（肽的增加豐度見(jiàn)圖1B;擴(kuò)展數(shù)據(jù)表2）。另一方面，肽段的減少則表明，在添加ADEP時(shí)存在的特定降解產(chǎn)物，可以被ADEP/CLPP進(jìn)一步降解，因此與本研究相關(guān)性較小。 |
3樓2014-10-23 23:01:47
ssssllllnnnn
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4樓2014-10-24 10:04:28
raohuihua
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5樓2014-10-25 00:49:08
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