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Actinomycetes have been prolific sources of novel secondary metabolites with a range of biologi- cal activities that may ultimately find application as therapeutic compounds. Hence several drug discovery companies are engaged in isolation of novel bioactive metabolites from these microbial sources. Antibiotics form the major class of such bioactive metabolites and have been widely used for treating infectious diseases. One of the most critical problems in clinical practice is the in- crease of prevalence of drug resistant strains, especially azole resistance among fungi. Due to this, there is a constant need for development of new antifungal antibiotics having novel scaffolds and/or mechanism of action. In our in-house screening program in the quest of novel and superior antifungal compounds, an actinomycetes strain PM0525875 was isolated from a marine inverte- brate. The extracts of this microbe showed potent in-vitro antifungal activity against drug resis- tant fungal strains. The antifungal active peak from the extract obtained by shake flask fermenta- tion was identified by chromatographic and other analytical techniques during bioactivity guided isolation. Later the fermentation conditions were optimized in 30 L fermentor for the production of sufficient amount antifungal compound for complete structural characterization. Consequently the fermented broth extract was subjected to bioactivity-guided fractionation, to isolate the active principle using different preparative chromatographic techniques followed by its characterization. The active principle was characterized to be Caerulomycin A. Minimum inhibitory concentration (MIC) of the compound was found in the range of 0.39 - 1.56 μg/ml against pathogenic fungal test strains. The phylogenetic analysis of producer strain using 16S rRNA sequence showed closest match with Actinoalloateichus cyanogriseus. Herewith we report the isolation of Caerulomycin A from marine invertebrate-associated Actinoalloteichus sp. using optimized medium and fermenta- tion conditions. |
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木蟲 (正式寫手)
- 翻譯EPI: 40
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★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
bxzc123: 金幣+20, 翻譯EPI+1, ★★★很有幫助 2014-10-26 21:26:58
bxzc123: 金幣+20, 翻譯EPI+1, ★★★很有幫助 2014-10-26 21:26:58
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有諸多生物活性可能最終應(yīng)用于治療的化合物，含有活性次生代謝物的放線菌資源豐富。因此，一些藥物研發(fā)公司致力于從這些微生物的資源中分離活性次生代謝產(chǎn)物。 抗生素是這種活性次生代謝產(chǎn)物的主要成分并被廣泛的用于治療傳染性疾病，在醫(yī)療實踐中最主要的問題之一就是普遍增長的耐藥性菌株，特別是對于菌類中唑類藥物的耐藥性。正因為此，對于起主導(dǎo)地位和作用機(jī)制的新抗真菌抗生素的研究一直都是必要的。在我們內(nèi)部對于新的和優(yōu)等的抗真菌的化合物的篩選項目中，從海洋無脊椎動物中分離出了放線菌菌株 PM0525875 |
2樓2014-10-26 19:51:25
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木蟲 (正式寫手)
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- 注冊: 2012-09-22
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- 專業(yè): 木材科學(xué)與技術(shù)
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bxzc123: 金幣+35, ★★★很有幫助 2014-10-26 21:27:03
bxzc123: 金幣+35, ★★★很有幫助 2014-10-26 21:27:03
| 這種細(xì)菌的抽提物表明體外抗真菌性能在抑制真菌菌株的耐藥性是有效的。使用搖瓶發(fā)酵法，生物活性物的分離采用色析法和其他分析分析方法，從抽提物中獲得的抗真菌峰被識別出來。然后對于足量的抗真菌化合物的生產(chǎn)，進(jìn)行結(jié)構(gòu)表征最佳的發(fā)酵條件為在30L的發(fā)酵罐中。因此，發(fā)酵液體培養(yǎng)基抽提物采用活性導(dǎo)向分餾法，根據(jù)其描述，使用不同的色譜技術(shù)去分離有效成分�；钚猿煞种幸詼\藍(lán)霉素A為主，對于抵抗致病真菌實驗株，化合物最低抑菌濃度范圍在0.39--1.56 μg/ml 之間。使用16S rRNA序列方法，菌株產(chǎn)生菌的種系進(jìn)化分析表明和Actinoalloateichus cyanogriseus.最為接近。因此，我們研究出最佳媒介和發(fā)酵條件，從海洋無脊椎與Actinoalloteichus相近的屬中分離出淺藍(lán)霉素A |
3樓2014-10-26 20:40:13
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